Q175 Knock-In Mouse 

The Q175 knock-in mouse contains human mHtt allele with the expanded CAG repeat (~179 CAG repeats) within the native mouse huntingtin gene. Thus, this model is more representative of the human disease from a genetic aspect. It adds value for longitudinal studies for chronic treatment effects assessed by motor behavior, cognitive assays, and in vivo MRI and MRS during the course of the study. All studies can be customized according to client needs.

The Q175 mouse shows robust, gene dose-dependent, progressive, and early-onset alterations in the validated endpoint assays. This model offers a good window of opportunity to study chronic compound effects. Both homo- and heterozygous Q175 mice exhibit first signs of motor symptoms early, from 3-4 months of age, and behavioral deficits are accompanied by marked brain atrophy and brain metabolite changes by 8 months.

Example Study Paradigm using the Q175 Knock-in Mouse Model of Huntington's Disease 

Click here to see a diagram outlining an example study paradigm using the Q175 knock-in mouse model of Huntington's disease.

 Q175 Mouse Study Components

• Motor Deficits
• Open field test
• Rear climbing

• Cognitive Deficits
• Procedural two-choice swim test
  
• Neurological Index
  
• Imaging Tools
• In vivo MRI for Brain Volumetry (whole brain, cortex, striatum)
• In vivo 1H-MRS of the Striatum Metabolic Profile
• Biomarkers
• Ask for various options in mRNA and protein detection assays

For additional information on our Huntington's disease research studies with the Q175 knock-in mouse model, please contact us at askcharlesriver@crl.com.