In Vitro CNS Models | Charles River Discovery Services

Neurodegeneration 

Primary cultures offer fast access to study molecules of interest directly in relevant cell types. Primary cultures are real cells present in tissue instead of immortalized cell lines, and are good tools for lead molecule identification (efficacy and toxicity screening). Data is available within few weeks from study initiation.

Model for Neurodegeneration

  • Client compounds and BDNF in cortical/hippocampal cultures


Ability to promote growth of neurites (Neurite outgrowth, NOG) is an important feature for compounds studied for CNS degenerative diseases (e.g., stroke, Alzheimer’s disease). Primary cortical and hippocampal cultures outgrow actively and the effect of compounds on a number of processes and branches as well as on the length of neurites can be studied.

Diagram outlining an example study paradigm using our in vitro model of neurodegeneration

 

Click here to see a diagram outlining an example study paradigm using our in vitro model of neurodegeneration.

 


Data

Mean Process Number

 

 

Mean Process Number

 

 

Mean Process + Branch Number

 

 

Mean Process + Branch Number

 

 

Mean Total Length of Processes + Branches

 

 

Mean Total Length of Processes + Branches

 

MAP-2 -immunocytochemistry on cortical cultures

MAP-2 -immunocytochemistry on cortical cultures

For more information about our in vitro neurodegeneration model, please contact us at 1.877.CRIVER.1 or askcharlesriver@crl.com.

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For more information, contact us at:

1.877.CRIVER.1 (1.877.274.8371)
askcharlesriver@crl.com

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