Charles River Now Offers MucilAir™ Human Airway Toxicity Test
Apr 24 2012
MucilAir™ was evaluated for use in predicting upper airway toxicity by treating tissues with increasing concentrations of sodium dodecyl sulfate (SDS). Evaluations were performed to assess monolayer integrity (transepithelial electrical resistance [TEER]), membrane integrity (lactate dehydrogenase [LDH] release), metabolic competence (resazurin metabolism) and inflammatory mediator (IL-8) release. Histology and scanning electron microscopy (SEM) were used to assess morphology.
When treated with SDS, it was concluded that MucilAir™ displayed dose-dependent responses, with cytotoxicity visualized by histology at ≥0.6 mM and measured chemically at ≥1.25 mM. Monolayer integrity (TEER) and marker release (LDH and IL-8) patterns were comparable between equivalently dosed units. These data support the use of MucilAir™ as a relevant model for airway toxicity studies.
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