Now Available – Reporter Mice: A Way to Study Molecular Processes In Vivo
October 26, 2012 - Charles River Genetically Engineered Models and Services (GEMS) is now offering three new mouse models through our collaboration with Transgenic Operative Products (T.O.P.) srl. Receive an evaluation order of the T.O.P. reporter mice as part of the Charles River Model Evaluation Program, implemented to provide a risk-free, cost-effective way of determining if our models are right for your research. To place your order, complete our Model Evaluation Request Form.
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repTOP™ mitoIRE: for researching proliferative effects in oncology, regenerative medicine and toxicology
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repTOP™ ERE-Luc: for assessing stimulation and activity of the estrogen receptors, with primary areas of research in reproductive functions and dysfunctions, menopause and related diseases, and hormone-regulated neurological disorders (depression and neurodegeneration)
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repTOP™ PPRE-Luc: for study of peroxisome proliferation in diabetes, arteriosclerosis, inflammation, lipid metabolism disorders and cardiovascular disease
Luciferase genes (the reporter) have been engineered to be under the control of three different promoters which allow the in vivo imaging of physiological and pathological processes using a noninvasive technique. Coupled with a low signal-to-noise ratio, good reproducibility and high specificity, these models offer a versatile way to quantitatively assess both endogenous processes, such as estrogen receptor activity during the estrous cycle (repTOP™ ERE-Luc); toxicological/pathological endpoints, such as tumor development over time (repTOP™ mitoIRE); or study the role of Peroxisome Proliferator Activated Receptors (PPARs) in Amytrophic Lateral Sclerosis by crossing with hSOD1G93A mice (repTOP™ PPRE-Luc).
The noninvasive nature of the bioluminescent readout allows for multiple readouts for both short-term and long-term studies in the same animal, thus minimizing inter-animal variation and potentially using fewer animals per study. In some cases, such as in the induction of skin cancer, the animal can act as its own control, with vehicle being administered on one side and the tumorigenic agent on the other.
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