antisera complete production packages
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Complete Package I
- Peptide synthesis (70–95% purity, 5–10 mg, up to 15 AS)
- Support during peptide selection (ProteanTM sequence analysis)
- Conjugation to KLH, BSA, or OVA (2 mg peptide, 20 mg KLH)
- 2 SPF rabbits, 70-day protocol
- 4 immunization injections per rabbit
- 1 pre-immunization serum (pre-bleed), 2 test sera (1. and 2. test bleed), final bleed (approximately 160 mL)
Complete Package II
- Peptide synthesis (70–95% purity, 5–10 mg, up to 15 AS)
- Support during peptide selection (ProteanTM sequence analysis)
- Conjugation to KLH, BSA, or OVA (2 mg peptide, 20 mg KLH)
- 2 SPF rabbits, 70-day protocol
- 4 immunization injections per rabbit
- 1 pre-immunization serum (pre-bleed), 2 test sera (1. and 2. test bleed), final bleed (approximately 160 mL)
- Serum titer control by ELISA
Complete Package III
- Peptide synthesis (70–95% purity, 5–10 mg, up to 15 AS)
- Support during peptide selection (ProteanTM sequence analysis)
- Conjugation to KLH, BSA, or OVA (2 mg peptide, 20 mg KLH)
- 2 SPF rabbits, 70-day protocol
- 4 immunization injections per rabbit
- 1 pre-immunization serum (pre-bleed), 2 test sera (1. and 2. test bleed), final bleed (approximately 160 mL)
- Serum titer control by ELISA
- Development of HYDRA® peptide immunoaffinity purification column (1 mL matrix)
- Immunoaffinity isolation of monospecific antibody from 50 mL serum
- Sequential elution of 3 fractions of different affinity
- Purification control of monospecific antibody
- Quality control of monospecific antibody by ELISA
- Detailed documentation, chromatograms
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antisera production, peptide synthesis, peptide immunoaffinity purification column, monospecific antibody
Antisera Production Services | Polyclonal Antibodies | Charles River
Charles River develops and produces polyclonal antibodies in rabbits, guinea pigs, as well as other species. Clients can choose an all-inclusive service package or single modules covering each step necessary to achieve high-quality polyclonal antibodies.