BASIC PACKAGE
- Immunization*
Immunization of 3 BALB/c mice with 4 injections within 3 months; initial screening and evaluation of serum titer by ELISA
- Fusion
Spleen cell preparation from animal with the best response against the antigen, fusion, distribution of hybridoma cells into 96-well plates, screening of hybridomas - 10 plates total
- Analysis
Detection of specific antibody in culture medium, ELISA with 1 antigen, delivery of 20 culture supernatants of hybridoma cells with the best response against the antigen; cultivation of 5 positive clones, delivery of 5 vital or cryopreserved clones
*If no animal produces sufficiently high titers due to low immunogenicity of the antigen, the production process can be ended here without further costs.
STANDARD PACKAGE
- Immunization*
Immunization of 3 BALB/c mice with 4 injections within 3 months; initial screening and evaluation of serum titer by ELISA.
- Fusion
Spleen cell preparation from animal with the best response against the antigen, fusion, distribution of hybridoma cells into 96-well plates, screening of hybridomas - 20 plates total
- Analysis
Detection of specific antibody in culture medium, ELISA with 1 antigen, delivery of 30 culture supernatants of hybridoma cells with the best response against the antigen; cultivation of 10 positive clones, delivery of 10 vital or cryopreserved clones
*If no animal produces sufficiently high titers due to low immunogenicity of the antigen, the production process can be ended here without further costs.
For technical support or to place an order for antibody production services, please email us at antibody@crl.com.