Tg2576 mice overexpress a mutant form of amyloid precursor protein (APP), APPK670/671L, linked to early onset familial Alzheimer’s disease (AD), developing amyloid plaques and progressive cognitive deficits. Charles River Discovery Services’ AD platform with the Tg2576 mouse includes cognitive testing, measurement of the levels of soluble and insoluble beta amyloid, and immunohistochemical measurements. All studies can be customized according to client needs.
The Tg2576 mice show impaired cognitive functions in the contextual fear conditioning test, coinciding with the increased cortical and hippocampal soluble beta amyloid levels starting from 5 months of age. The increased insoluble beta amyloid levels and the amyloid plaques are evident at 13 months of age.
Behavioral and Cognitive Tests with the Tg2576 Mouse
Motor Functions
- Open Field Test
- Total distance traveled
- Distance traveled in the center area
- Total rearing frequency
- Rearing rate in the center area
- Average velocity
- Multiple other variables
Cognitive Deficits
- Contextual Fear Conditioning Test (CFC)
- Memory for context
- Memory for cue
Amyloid Beta ELISA and Immunohistochemistry in Tg2576 Mice
Measurements of beta amyloid (Aβ) levels
- 5 months of age
- soluble Aβ1-40 and 1-42 from cortex and hippocampus
- soluble Aβ1-42 from plasma and cerebrospinal fluid (CSF)
- 13 months of age
- soluble and insoluble Aβ1-40 and 1-42 from cortex and hippocampus
- soluble Aβ1-40 and 1-42 from plasma and CSF
- Plaque load in cortex and hippocampus at 13 months of age
Data
Hippocampal Aβ1-42 in 5-Month-Old Tg2576 Mice
Cortical Soluble Aβ1-40 Levels in Tg2576 Mice
Cortical Soluble Aβ1-42 Levels in Tg2576 Mice
Immunohistochemistry in the Tg2576 Mice
Immunohistochemistry and other measurements
- Astro- and Microgliosis by GFAP and ED-1 IHC
- Cytokine expression by IHC and/or ELISA (IL1beta, IL-6)
- Cell proliferation (Neurogenesis) by BrdU and IHC with Stereology (MAP-2, GFAP)
- IDE activity assay
- Phospho-GSK3beta assay
- Synaptophysin WB
- C99, C83 and holoAPP WB
- Neurochemical profile by anesthetized/awake microdialysis (ACh, 5HT, NE, DA, Glu, metabolites)
- Plasma, Cerebral tissue and Microdialysate samples for PK and/or Neurochemicals
For more information about our Alzheimer’s disease research studies with the Tg2576 mouse, contact us at 1.877.CRIVER.1 or askcharlesriver@crl.com.
Charles River also offers an in vitro Alzheimer’s disease model that is created by exposing a cortical/hippocampal cell culture to Aβ1-42. For more information, click here.