We understand how challenging it is to develop novel anticancer therapies. Charles River can facilitate your selection of the most active agent and the most appropriate dose regimens in a variety of xenograft and genetically engineered mouse models of tumor.
Using various in vitro and in vivo tumor models, Charles River’s Oncology Services are available to help customers evaluate novel anticancer therapies. We offer human xenograft and murine syngeneic models bearing tumor cell lines that are either obtained from commercial sources or provided to us directly from the customers. Our research scientists will also work closely with you to develop detailed study protocols to meet your project goals.
In Vitro Compound Efficacy Testing
This service involves the evaluation of cell proliferation and cell death in response to test article administration in cultured human or murine tumor cell lines.
Tumor Injection Service
Most frequently, tumor cells are implanted via subcutaneous injection into the flank of nude mice.
- Human tumor xenograft models are generated by inoculating a human tumor cell line into an immunodeficient rodent.
- Syngeneic tumor models are created by inoculating a mouse or rat tumor cell line into the corresponding immunocompetent mouse or rat strain.
Once the implanted tumors reach a predetermined volume, we can transport the animal models to our customers ready for study (inject and ship) or perform compound efficacy studies under contract. Our tumor injection services can assist you in more optimal resource allocation.
In Vivo Compound Efficacy Studies
In an appropriate animal model, one or more therapeutic compounds may be administered to assess efficacy against tumor induction, latency, proliferation, and metastasis. Subcutaneous models provide a consistent and reproducible tool for evaluating tumor cell growth, as well as permit easy access to the mass for tumor treatment and ultrasound evaluation. Metastatic tumor incidence can be monitored through radiography, high-resolution ultrasound imaging, and necropsy. Customers have the option to receive raw data and analyzed data at the completion of a study. Ancillary laboratory services such as necropsy, histopathology, and molecular phenotyping (i.e., mRNA and protein expression patterns) are also available to help maximize data capture.