In Vitro CNS Models | Charles River Discovery Services

Alzheimer's Disease 

Primary cultures offer fast access to study molecules of interest directly in relevant cell types. Primary cultures are real cells present in tissue instead of immortalized cell lines, and are good tools for lead molecule identification (efficacy and toxicity screening). Data is available within few weeks from study initiation.

In vitro Model for Alzheimer's Disease

  • Abeta1-42 exposure in cortical/hippocampal culture


In Alzheimer’s disease, the cortex and hippocampus are the most affected brain areas that are seriously damaged by Amyloid-beta (Aβ). These target cells can be cultured and exposed to the toxic form, Aβ
1-42. Cell death is determined by LDH release and immunocytochemical evaluation of viable cells.

Diagram outlining an example study paradigm using our in vitro Alzheimer's disease model


Click here to see a diagram outlining an example study paradigm using our in vitro Alzheimer's disease model.

 

 

Data 

LDH Aβ1-42 in Cortical Cultures

 

 

  LDH Aβ1-42 in Cortical Cultures

 

NeuN-IR Cells Aβ1-42 in Cortical Cultures

 

 

NeuN-IR Cells Aβ1-42 in Cortical Cultures

 

NeuN Immunocytochemistry on Hippocampal Cultures

NeuN Immunocytochemistry on Hippocampal Cultures


For more information about our in vitro models to support Alzheimer’s disease research, please contact us at 1.877.CRIVER.1 or askcharlesriver@crl.com.

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For more information, contact us at:

1.877.CRIVER.1 (1.877.274.8371)
askcharlesriver@crl.com

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