Primary cultures offer fast access to study molecules of interest directly in relevant cell types. Primary cultures are real cells present in tissue instead of immortalized cell lines, and are good tools for lead molecule identification (efficacy and toxicity screening). Data is available within few weeks from study initiation.
Models for Stroke and Ischemia
The most important components of tissue damage in stroke are deprivation of oxygen and glucose, excitotoxicity (excess release of glutamate from affected cells) and production of harmful free radicals. Charles River Discovery Services’ in vitro model battery for stroke provides access to all these mechanisms involved in stroke. The possible exposures include:
- Oxygen-glucose deprivation
- Glutamate-induced excitotoxic insult
- Hydrogen peroxide-induced free radical insult
Click here to see a diagram outlining an example study paradigm using our in vitro models of stroke and ischemia.
Data
Oxygen-glucose deprivation
NeuN-IR Cells OGD in Cortical Cultures
LDH OGD in Cortical Cultures
Glutamate exposure
LDH Glutamate in Cortical Cultures
Cell Count Glutamate in Cortical Cultures
H2O2 exposure
LDH H2O2 in Cortical Cultures
Cell Count H2O2 in Cortical Cultures
MAP-2 -immunocytochemistry on mixed cortical cultures
For more information about our in vitro stroke and ischemia models, please contact us at 1.877.CRIVER.1 or askcharlesriver@crl.com.