Embryology | Genetically Engineered Models & Services | Charles River

cryopreservation 

Storage Straws - All embryos and sperm cryopreseved by Charles River are loaded in straws and stored in liquid nitrogen 

At Charles River, we know that mouse and rat cryopreservation provides a permanent archive of your valuable genetically engineered strains to ensure that you will be able to recover live animals at any future date. Cryopreservation of embryos and/or sperm provides an effective way to create a low-cost backup to your live animal colony in the event of a colony disaster and/or store lines that are no longer actively being used. With over twenty years of experience and the knowledge gained from cryopreserving over 10,000 unique genetically engineered lines, our expertise helps ensure that your backup will be there when you need it.

Our service options include:

Mouse Cryopreservation Services

Service Deliverables
Mouse
Cryopreservation I
250 - 300 embryos.
Animal requirements: 4-6 males
Mouse
Cryopreservation II
250 - 300 embryos. Includes expansion of a homozygous colony to provide embryo donors.
Animal requirements: 5-10 breeding pairs
Mouse Sperm Cryopreservation ~15 straws of sperm. Includes pre- and post-thaw sperm analysis.
Animal requirements: 2 males
I•CRYO A do-it-yourself kit to cryopreserve and QC 5 lines via sperm.
Rat Cryopreservation Services
Service Deliverables
Rat Cryopreservation I 250 - 300 embryos.
Animal requirements: 4-6 males
Rat Cryopreservation II 250 - 300 embryos. Includes expansion of a homozygous colony to provide embryo donors.
Animal requirements: 5-10 breeding pairs
Rat Sperm Cryopreservation ~15 straws of sperm. Includes pre- and post-thaw sperm analysis.
Animal requirements: 2 males

All cryopreserved germ plasm can be maintained at Charles River or shipped to your facility for storage. Embryos and sperm stored in our facilities are divided between two independent locations, each with its own automated liquid nitrogen filling system and alarm network to help ensure the safety of your cryopreserved material.

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For more information, contact us at:

1.877.CRIVER.1 (1.877.274.8371)
askcharlesriver@crl.com

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Detailed information about our cryopreservation services and procedures.
When you're ready to start a project, complete this form and our project management staff will take care of the rest.

News & Events

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May 20 - Phenotype and Genotype Comparison ...
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Jun 21-Jun 25 - Chicago, IL - American Diabetes Association’s 73rd Scientific Sessions
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Charles River Laboratories, Inc.