ORIGINThe 11BHSD2 mouse was produced by injection of a construct, containing 11β-HSD2 cDNA isolated from mouse kidney and cloned into plasmid Clone 26 to recruit the 5.4 kb mouse α–myosin heavy chain (α-MHC) promoter, into pronuclei of C57BL/6 embryos. Transgenic mice were maintained by sibling mating and used as hemizygotes. Transferred to Charles River under exclusive license from Pfizer, Inc., in 2011.
STRAIN CODEComing Soon
MUTATION INFORMATIONThis model was developed to evaluate the role of aldosterone in cardiac pathophysiology by producing over expression of 11β-HSD2 in cardiac myocytes, leading to aldosterone selectivity in these cells. In one founder line (326).Key features of this model include:
Aldosterone is known to regulate blood pressure and blood volume via unidirectional transepithelial sodium transport. Additionally, other direct roles of aldosterone in the cardiovascular system have been postulated. In experimental models of hypertension, aldosterone produces blood pressure elevation by stimulating brain mineralocorticoid receptors (MRs), and when combined with high salt intake, produces cardiac hypertrophy, fibrosis and vascular inflammation injury independent of blood pressure elevation. Expression of 11β-hydroxysteroid dehydrogenase type 2, an enzyme found in various epithelial cells and vascular smooth muscle cells, converts endogenous glucocorticoid to their 11-keto forms, which are receptor-inactive, thereby conferring selectivity towards aldosterone to these cells. In cardiac myocytes, the endogenous levels of 11β-HSD2 is low and, thus, aldosterone plays only a minor role in activation of MRs under normal physiological conditions.
RESEARCH APPLICATIONCardiac Hypertrophy, Heart Failure
ORDERING INFORMATIONPlease call 1.800.LAB.RATS if you would like to place an order.
For additional information about 11BHSD2 mice, please contact Technical Services at 1.877.CRIVER.1 (1.877.274.8371) or firstname.lastname@example.org.
DISCLAIMER11BHSD2 mice may be subject to the licensed patents and are sold only for research purposes under agreement from Pfizer, Inc.
For more information, contact us at:
*indicates a required field.
The information you provided will be kept confidential.
Clinical pathology data can assist you in selecting the most appropriate model for your study and can provide important information on the physiology and health of your research animals.
We are now the exclusive distributor of the TARGATT™ mouse. The TARGATT mouse is a transgenic mouse designed to produce animal models with investigator designed exogenous DNA sequences knocked-in to a specific site.
Jun 10–Jun 13
Visit us in booth #091 to learn more about our recent innovations including expanded diagnostic capabilities, a new online portal connecting clients with their colonies and a deep-rooted commitment to humane care.
May 28–Jun 01
The International Bone and Mineral Society (IBMS) and the Japanese Society for Bone and Mineral Research (JSBMR) are excited to sponsor the second joint scientific meeting by the two organizations.
May 27–May 29
Charles River and the René Remie Surgical Skills Centre (RRSSC) have joined their expertise and are pleased to offer a 3-day surgery training course. Held at the RRSSC in the Netherlands, this training is designed to be very practical and combines lectures with hands-on exercises, ensuring you receive the most comprehensive training experience. This course is recommended for simple procedures only (e.g., simple exeresis, simple catheterizations such as jugular vein or femoral vein, simple ALZET pump implantation, ECG radiotelemetry).
This position is responsible for serving as a as a study director (SD), project scientist (PS) and/or principal investigator (PI) in the direction and execution of assigned studies in compliance with GLP regulations as they apply to the conduct of nonclinical research; coordinating all phases of the study planning process with appropriate departments; generating high-quality project plans, protocols, amendments and reports appropriate for assigned studies; reviewing, interpreting, integrating and presenting data on assigned studies; and functioning as contact for the planning and execution of sponsor interaction related to assigned studies, including proposal management and study scheduling, conduct and reporting.
This position is responsible for managing international aspects of US Federal income tax compliance; assisting with international tax planning, including identification of planning opportunities, designing and implementing plans, and transfer pricing process; overseeing quarterly ETR forecast process; and participating in the tax provision process, including assessment of tax risks, documentation of key positions, and review of non-US tax provision submissions.
Charles River Laboratories, Inc.