ORIGINAt the Ludwig Institute for Cancer Research, a hybrid construct containing H-2Kb (MHC Class I antigen) 5' promotor sequences fused to the early region of the SV40 mutant tsA58, which encodes both the large and small SV40 tumor antigens, was microinjected into fertilized oocytes from CBA/Ca x C57BL/10 F1 mice. Following reimplantation, 88 mice were born, of which 34 were transgenic and carried one to five copies of the gene. RNA from a variety of tissues from one nontransgenic and three transgenic animals was analyzed by Northern blot analysis using a SV40 early region-specific probe. RNA extracted from tissues of transgenic mice contained varying amounts of a 2.5kb RNA species, while no tsA58TAg RNA was detected in tissues of the nontransgenic mouse; thymus and liver showed the highest level of expression, while brain showed the lowest. Distribution rights to Charles River in 1991.
STRAIN CODE237 (HOMOZYGOUS)238 (HETEROZYGOUS)
COAT COLORPrimarily agouti, infrequently black
TRANSGENE INFORMATIONConstruction of this transgene involved fusion of the 5'-flanking promoter sequences as well as the transcriptional initiation site of the H-2Kb Class 1 gene to the tsA58 early region coding sequences. The 4.2kb EcoRI to NruI fragment encompassing the H-2Kb promoter sequences was ligated to the 2.7kb BglI to BamHI fragment derived from tsA58 early region and pUC19 double digested with EcoRI and BamHI. The Bg1I site had been blunted using the Klenow fragment of E. coli DNA Polymerase1 to allow fusion to the Nru1 site. For purposes of microinjection, the H-2KbtsA58 DNA fragment was isolated free of vector sequences by digestion with EcoRI and SalI.
ORDERING INFORMATIONPlease call 1.800.LAB.RATS if you would like to place an order.
For additional information, please contact Technical Services at 1.800.338.9680 or email@example.com.
For more information, contact us at:
*indicates a required field.
The information you provided will be kept confidential.
Clinical pathology data can assist you in selecting the most appropriate model for your study and can provide important information on the physiology and health of your research animals.
We are now the exclusive distributor of the TARGATT™ mouse. The TARGATT mouse is a transgenic mouse designed to produce animal models with investigator designed exogenous DNA sequences knocked-in to a specific site.
Jun 10–Jun 13
Visit us in booth #091 to learn more about our recent innovations including expanded diagnostic capabilities, a new online portal connecting clients with their colonies and a deep-rooted commitment to humane care.
May 28–Jun 01
The International Bone and Mineral Society (IBMS) and the Japanese Society for Bone and Mineral Research (JSBMR) are excited to sponsor the second joint scientific meeting by the two organizations.
May 27–May 29
Charles River and the René Remie Surgical Skills Centre (RRSSC) have joined their expertise and are pleased to offer a 3-day surgery training course. Held at the RRSSC in the Netherlands, this training is designed to be very practical and combines lectures with hands-on exercises, ensuring you receive the most comprehensive training experience. This course is recommended for simple procedures only (e.g., simple exeresis, simple catheterizations such as jugular vein or femoral vein, simple ALZET pump implantation, ECG radiotelemetry).
This position is responsible for serving as a as a study director (SD), project scientist (PS) and/or principal investigator (PI) in the direction and execution of assigned studies in compliance with GLP regulations as they apply to the conduct of nonclinical research; coordinating all phases of the study planning process with appropriate departments; generating high-quality project plans, protocols, amendments and reports appropriate for assigned studies; reviewing, interpreting, integrating and presenting data on assigned studies; and functioning as contact for the planning and execution of sponsor interaction related to assigned studies, including proposal management and study scheduling, conduct and reporting.
This position is responsible for managing international aspects of US Federal income tax compliance; assisting with international tax planning, including identification of planning opportunities, designing and implementing plans, and transfer pricing process; overseeing quarterly ETR forecast process; and participating in the tax provision process, including assessment of tax risks, documentation of key positions, and review of non-US tax provision submissions.
Charles River Laboratories, Inc.