ORIGINThe transgene was constructed by inserting four copies of the chicken chromatin insulator sequence into the multiple cloning region of the pHelix1. An expression cassette fragment consisting of the neuron-specific enolase (NSE) promoter, a random filler sequence, and the SV40 polyadenylation signal sequence was then subcloned into a blunted Bam H1site on the pHelix/CI vector such that it was flanked on each side by two copies of the chromatin insulators. The G100L MMP-9 cDNA was subcloned as a Xba1fragment into the NHe1 and SPe1sites of the vector, replacing some of the random filler sequence. The transgene was purified using Elutip columns and used for pronuclear injection into FVB/N embryos, which were then transferred to pseudopregnant CD-1 females. Founder transgenics were identified by PCR using tail tissue. Founder mice were bred to FVB/N mates and transgene-positive offspring were used to maintain the individual transgenic lines. Transferred to Charles River under exclusive license in 2011.
STRAIN CODEComing Soon
MUTATION INFORMATIONMatrix Metalloproteinases (MMPs) are expressed and secreted from cells as inactive zymogens, the latency of which are maintained through a cysteine switch mechanism in the highly conserved region of the propeptide. Due to the tight regulation of enzyme activity, through a two-step process, transgenic animals overexpressing MMP-9 may give rise to increased expression of the latent enzyme, but not the active enzyme. By designing mutant forms of the enzyme that autocatalytically removed the prodomain to yield active enzyme, the effects of MMP-9 in the CNS can be studied. Multiple mutations were made by site-directed mutagenesis and several mutations disrupted the function of the cysteine switch mechanism, leading to dysregulation of MMP-9.
RESEARCH APPLICATIONInflammation, extracellular remodeling
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DISCLAIMERMMP9 mice may be subject to the licensed patents and are sold only for research purposes under agreement from Pfizer, Inc.
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Clinical pathology data can assist you in selecting the most appropriate model for your study and can provide important information on the physiology and health of your research animals.
We are now the exclusive distributor of the TARGATT™ mouse. The TARGATT mouse is a transgenic mouse designed to produce animal models with investigator designed exogenous DNA sequences knocked-in to a specific site.
Jun 10–Jun 13
Visit us in booth #091 to learn more about our recent innovations including expanded diagnostic capabilities, a new online portal connecting clients with their colonies and a deep-rooted commitment to humane care.
May 28–Jun 01
The International Bone and Mineral Society (IBMS) and the Japanese Society for Bone and Mineral Research (JSBMR) are excited to sponsor the second joint scientific meeting by the two organizations.
May 23–May 23
This full-day seminar encompasses selected topics from our Charles River Short Course on Laboratory Animal Science. We would like to acknowledge Michigan State University for hosting this event and extend our heartfelt thanks to guest speaker Dr. Danielle Ferguson.
This position is responsible for serving as a as a study director (SD), project scientist (PS) and/or principal investigator (PI) in the direction and execution of assigned studies in compliance with GLP regulations as they apply to the conduct of nonclinical research; coordinating all phases of the study planning process with appropriate departments; generating high-quality project plans, protocols, amendments and reports appropriate for assigned studies; reviewing, interpreting, integrating and presenting data on assigned studies; and functioning as contact for the planning and execution of sponsor interaction related to assigned studies, including proposal management and study scheduling, conduct and reporting.
This position is responsible for managing international aspects of US Federal income tax compliance; assisting with international tax planning, including identification of planning opportunities, designing and implementing plans, and transfer pricing process; overseeing quarterly ETR forecast process; and participating in the tax provision process, including assessment of tax risks, documentation of key positions, and review of non-US tax provision submissions.
Charles River Laboratories, Inc.