As a poultry producer, you need to transport poultry and egg products across state lines in accordance with the USDA National Poultry Improvement Plan (NPIP) to avoid disease transmission to other poultry populations. Charles River Avian Vaccine Services makes it easier for you to safeguard your products from that contingency by offering USDA-licensed Mycoplasma gallisepticum, Mycoplasma synoviae, Mycoplasma meleagridis, and Salmonella pullorum antigens for serum plate agglutination (SPA) testing. These antigens have been standardized against reference antigens and known positive and negative sera. With them, you can feel confident that your avian products conform to the NPIP standards.
USDA-Licensed Plate Antigens
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Mycoplasma gallisepticum Antigen (USDA PRODUCT CODE: 5071.02)
SPAFAS MG Plate Antigen for Plate Agglutination Test
(Catalog No. 10100760)
This antigen is a suspension of killed, stained A5969 strain of Mycoplasma gallisepticum (MG) for serum plate agglutination (SPA) testing of chicken and turkey flocks. |
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Mycoplasma meleagridis Antigen (USDA PRODUCT CODE: 5072.01)
SPAFAS MM Plate Antigen for Plate Agglutination Test
(Catalog No. 10100825)
This antigen is a suspension of killed Mycoplasma meleagridis (MM) for serum plate agglutination (SPA) testing of turkey flocks. |
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Mycoplasma synoviae Antigen (USDA PRODUCT CODE: 5073.02)
SPAFAS MS Plate Antigen for Plate Agglutination Test
(Catalog No. 10100761)
This antigen is a suspension of killed, stained WVU1853 strain of Mycoplasma synoviae (MS) for serum plate agglutination (SPA) testing of chicken flocks. |
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Salmonella pullorum Antigen (USDA PRODUCT CODE: 5207.00)
LAH Pullorum Stained Antigen for Plate Agglutination Test
(Catalog No. 10100762)
This antigen is a suspension of five (5) killed, stained strains (4, 11, 77, 79, and 296) of Salmonella pullorum for serum plate agglutination (SPA) testing of chicken and turkey flocks. |
Serum Controls
Positive and negative control serum antibodies are available for MG, MS, Salmonella polyvalent (Groups B & D), HPG, and Mycoplasma meleagridis.
SPA Testing Method
The serum plate agglutination (SPA) test is used to detect specific antibodies that will bind to an antigen and cause visible “clumping” or agglutination. The antigen is prepared from cultures and a dye is added to improve visibility of the reactions. A prescribed amount of antigen is placed on a solid support such as a plastic or glass plate or mirror, keeping each drop of antigen separate. An equal amount of test serum is placed next to the antigen and these are then mixed together. After a short (2 minute) incubation, the mixture is examined for evidence of agglutination, which appears as discrete clumps of the stained particles with a clearer background. If no antibodies are detected, the mixture will remain opaque. The SPA is a screening test and positive reactions should be confirmed by other methods, such as Hemagglutination Inhibition or isolation of the organism from appropriate samples.
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