Charles River offers molecular pathology services that include in situ hybridization (ISH), real-time PCR (rt-PCR), and quantitative PCR (Q-PCR). Our staff has a strong background in molecular biology and histopathology. This integration means we can relate gene expression to tissue histomorphology in both normal tissues and therapeutic models of disease, providing our clients with valuable functional genomics information. The end result is the best possible interpretation and troubleshooting of molecular-based tools applied to toxicological pathology issues.
Charles River performs both isotopic and non-isotopic in situ hybridization for cell-specific localization of expressed genes in frozen and paraffin-embedded tissues. The detection of DNA and mRNA by in situ hybridization allows us to localize target DNA and gene expression to specific cell types in tissue sections. This enables our pathologists to assess the toxicity, efficacy, and biological function of novel therapeutic compounds.
We provide a wide range of services in support of in situ gene expression studies, including:
- Procurement, embedding, and sectioning of study tissues
- Synthesis of DNA and RNA probes (isotopic and non-isotopic)
- Optimized hybridization
- Histological evaluation by board-certified pathologists
- Reporting with representative color photomicrographs