Ten New PI3-K Strains from the Ludwig Institute for Cancer Research Ltd. and University of Edinburgh Now Available Through Charles River

March 3, 2010 – Charles River is pleased to announce the availability of ten new research models. Nine of the strains were created jointly by the Ludwig Institute for Cancer Research Ltd. (LICR), developers of the popular Immortomouse® strain offered through Charles River, and the University of Edinburgh, while the additional strain was created exclusively by LICR. These new PI3-K strains will help expand our portfolio of licensed genetically engineered models.

Listed below are general details about the new PI3-K strains. The S1039A strain was developed exclusively by the LICR, while the others were developed jointly by LICR and the University of Edinburgh.

p110alpha D933A constitutive
Full strain name: p110alpha PI 3-kinase kinase dead (PIK3CA-D933A constitutive)
Therapeutic areas: Angiogenesis, metabolism, cancer
Publication: Foukas, L.C., et al. Critical role for the p110alpha phosphoinositide-3-OH kinase in growth and metabolic regulation. Nature.  441(7091), 366-70 (2006).
Abstract: http://www.nature.com/nature/journal/v441/n7091/full/nature04694.html

alpha flox
Full strain name: p110 alpha flox
Therapeutic areas: Angiogenesis, metabolism, cancer
Publication: Graupera, M., et al. Angiogenesis selectively requires the p110alpha isoform of PI3K to control endothelial cell migration. Nature.  453(7195), 662-6 (2008).
Abstract: http://www.nature.com/nature/journal/v453/n7195/full/nature06892.html

p110alpha WT + neo/LacZ reporter cassette
Full strain name: p110alpha PI 3-kinase active + neo/LacZ reporter cassette (PIK3CA WT + neo/LacZ reporter cassette)
Therapeutic areas: Angiogenesis, metabolism, cancer, neuronal study
Publication: Supplementary text from Foukas, L.C., et al. Critical role for the p110alpha phosphoinositide-3-OH kinase in growth and metabolic regulation. Nature.  441(7091), 366-70 (2006). See FigS1.pdf.
Abstract: http://www.nature.com/nature/journal/v441/n7091/full/nature04694.html

beta flox
Full strain name: p110 beta flox
Therapeutic areas: Angiogenesis, metabolism, cancer
Publication: Guillermet-Guibert, J., et al. The p110beta isoform of phosphoinositide 3-kinase signals downstream of G protein-coupled receptors and is functionally redundant with p110gamma. Proc. Natl. Acad. Sci. USA. 105(24), 8292-7 (2008).
Abstract: http://www.ncbi.nlm.nih.gov/pubmed/18544649

p110beta KI + cassette
Full strain name: p110beta PI 3-kinase dead + neoLacZ reporter cassette PIK3CB/p110beta KI +neo/LacZ reporter cassette
Therapeutic areas: Fertility, cancer
Publication: No publication currently available.

p110beta D931A constitutive
Full strain name: p110beta PI 3-kinase dead PIK3CB D931A constitutive
Therapeutic areas: Fertility, cancer
Publication: No publication currently available.

p110beta DEL
Full strain name: p110beta-DEL-21/22
Therapeutic areas: Fertility
Publication: No publication currently available.

p110delta WT + neo/LacZ reporter cassette
Full strain name: p110delta PI 3-kinase active + neo/LacZ reporter cassette PIK3CD  WT + neo/LacZ reporter cassette
Therapeutic areas: Angiogenesis, immunology, cancer, neuronal study
Publication: Eickholt, B.J., et al. Control of axonal growth and regeneration of sensory neurons by the p110delta PI 3-kinase. PLOS. 2(9), 1-9 (2007).
Abstract: http://www.ncbi.nlm.nih.gov/pubmed/17846664

p110delta D910A constitutive
Full strain name: PI 3-kinase delta kinase-dead (PIKC3D kinase-dead)
Therapeutic areas: Certain areas of immunity, including autoimmunity, B- and T-cell  function, allergy
Publication: Okkenhaug, K., et al. Impaired B and T Cell Antigen Receptor Signaling in p110delta PI 3-Kinase Mutant Mice. Science. 297(5583), 1031-4 (2002).
Abstract: http://www.sciencemag.org/cgi/content/abstract/297/5583/1031

S1039A constitutive
Full strain name: p110delta S1039A
Therapeutic areas: Immunology
Publication: No publication currently available.

To receive more information about these strains or any of our genetically engineered models, or to place an animal order, please contact us at 1.877.CRIVER.1 (1.877.274.8371) or askcharlesriver@crl.com.