antisera complete production packages 

Complete  Package I

• Peptide synthesis (70–95% purity, 5–10 mg, up to 15 AS)
• Support during peptide selection (Protean^TM sequence analysis)
• Conjugation to KLH, BSA, or OVA (2 mg peptide, 20 mg KLH)
• 2 SPF rabbits, 70-day protocol
• 4 immunization injections per rabbit
• 1 pre-immunization serum (pre-bleed), 2 test sera (1. and 2. test bleed), final bleed (approximately 160 mL)

Complete  Package II

• Peptide synthesis (70–95% purity, 5–10 mg, up to 15 AS)
• Support during peptide selection (Protean^TM sequence analysis)
• Conjugation to KLH, BSA, or OVA (2 mg peptide, 20 mg KLH)
• 2 SPF rabbits, 70-day protocol
• 4 immunization injections per rabbit
• 1 pre-immunization serum (pre-bleed), 2 test sera (1. and 2. test bleed), final bleed (approximately 160 mL)
• Serum titer control by ELISA

Complete  Package III

• Peptide synthesis (70–95% purity, 5–10 mg, up to 15 AS)
• Support during peptide selection (Protean^TM sequence analysis)
• Conjugation to KLH, BSA, or OVA (2 mg peptide, 20 mg KLH)
• 2 SPF rabbits, 70-day protocol
• 4 immunization injections per rabbit
• 1 pre-immunization serum (pre-bleed), 2 test sera (1. and 2. test bleed), final bleed (approximately 160 mL)
• Serum titer control by ELISA
• Development of HYDRA^® peptide immunoaffinity purification column (1 mL matrix)
• Immunoaffinity isolation of monospecific antibody from 50 mL serum
• Sequential elution of 3 fractions of different affinity
• Purification control of monospecific antibody
• Quality control of monospecific antibody by ELISA
• Detailed documentation, chromatograms