Primary cultures offer fast access to study molecules of interest directly in relevant cell types. Primary cultures are real cells present in tissue instead of immortalized cell lines, and are good tools for lead molecule identification (efficacy and toxicity screening). Data is available within few weeks from study initiation.
In vitro Model of Inflammation
- Bacterial lipopolysaccharide exposure in glial culture
Inflammation occurs after stroke and is an important player also in Multiple Sclerosis. Inflammation can be studied in cortical glial cultures with bacterial lipopolysaccharide (LPS) exposure, and important end points include nitrite, IL-1β and TNF-α.
Click here to see a diagram outlining an example study paradigm using our in vitro model of inflammation.
Data
IL-1β in Cortical Glia
TNF-α in Cortical Glia
Nitrite in Cortical Glia
GFAP immunocytochemistry on cortical cultures
For more information about our in vitro inflammation model, please contact us at askcharlesriver@crl.com.