Primary cultures offer fast access to study molecules of interest directly in relevant cell types. Primary cultures are real cells present in tissue instead of immortalized cell lines, and are good tools for lead molecule identification (efficacy and toxicity screening). Data is available within few weeks from study initiation.
Model for Neurodegeneration
- Client compounds and BDNF in cortical/hippocampal cultures
Ability to promote growth of neurites (Neurite outgrowth, NOG) is an important feature for compounds studied for CNS degenerative diseases (e.g., stroke, Alzheimer’s disease). Primary cortical and hippocampal cultures outgrow actively and the effect of compounds on a number of processes and branches as well as on the length of neurites can be studied.
Click here to see a diagram outlining an example study paradigm using our in vitro model of neurodegeneration.
Data
Mean Process Number
Mean Process + Branch Number
Mean Total Length of Processes + Branches
MAP-2 -immunocytochemistry on cortical cultures
For more information about our in vitro neurodegeneration model, please contact us at askcharlesriver@crl.com.