The creation of knock-out or knock-in mice from targeted embryonic stem (ES) cells is often a crucial first step in research. Charles River provides this service by injecting your ES cells into a host morulae or blastocyst. The ES cells will be expanded and grown on mouse embryo fibroblasts prior to injection. We employ the use of a XYCloneTM laser system for the creation of live mice. Approximately 80-100 eight-cell embryos will be injected and transferred into pseudopregnant CD-1 recipient mice. The resulting litters will be genetically screened for the presence of the gene or identified by differences in coat color. Typically three to five chimeric animals will be produced. The resulting chimeric animals will be shipped back to you or maintained here at Charles River for additional breeding and expansion. In addition to this service, mycoplasma testing and karyotyping of the cell lines can be performed. All animals are maintained in the security of an isolator, and you will be provided with a complete health report prior to any animals being shipped to your facility.
For more information or to obtain pricing, please contact us at gems@crl.com.
|
animal model creation, ES cell injection, embryonic stem cells, chimeric animals, cell line, gene targeting, germline, knock-out mice, knock-in mice, XYCloneTM laser system, mycoplasma testing, karyotyping
Embryonic Stem (ES) Cell Injection | Genetically Engineered Models & Services | Charles River
The creation of knock-out or knock-in mice from targeted embryonic stem (ES) cells is often a crucial first step in research.