research animal models

ORIGIN
Transgenic mice were produced by injecting an 11.8 kb (NotI fragment) human genomic GLUT 4 transgene including a 5.3 kb of 5’flanking sequence and an SV40 polyadenylation sequence into the pronuclei of embryos from matings of C57BL/KsJ-m+/+ males and superovulated females. The embryos were then transferred to pseudopregnant CD-1 females. Founder mice were identified by PCR of total genomic DNA to obtain two mice with the recessive obese phenotype (+db/+db) and two founders with the recessive misty coat color (m+/m+). Independent lines were established by backcrossing lean founder mice with C57BL/KsJ-m+/db+ mates. Transferred to Charles River under exclusive license in 2011.

STRAIN CODE
Coming Soon

COAT COLOR
Black

MUTATION INFORMATION
Non-insulin-dependent diabetes mellitus (NIDDM) is characterized by hyperglycemia in both fed and fasted states and is manifested by defects in both insulin secretion from the pancreas and insulin action in the peripheral tissue (liver, skeletal muscle and adipose tissue). The defect in transport cannot be attributed solely to the expression of the major transporter GLUT 4, as little or no reduction in GLU4 levels are observed in NIDDM patients. Db/db mice mimic the human disease, including hyperglycemia, insulin resistance and obesity, and show a marked decrease in skeletal muscle utilization without an accompanying decrease in GLUT 4 expression.

This model evaluates the effect of overexpression of GLUT 4 to reduce hyperglycemia and improve glycemic control.

Features of the Model

• High expression of GLUT4 was observed in cardiac muscle, hindquarter skeletal muscle, brain, perirenal brown adipose tissue and white adipose tissue.
• Not expressed in kidney, spleen or pancreas
• Marked reduction of fed and fasted plasma glucose levels compared to age-matched controls (db/db)
• Enhanced disposal of an oral glucose challenge test
• Elevated plasma membrane-associated GLUT 4 protein in the basal state, which increases markedly after an insulin/glucose challenge when compared to controls.

RESEARCH APPLICATION
Diabetes

ADDITIONAL INFORMATION
For additional information about GLUT 4 mice, please contact us at info@eu.crl.com.  

DISCLAIMER
GLUT 4 mice may be subject to the licensed patents and are sold only for research purposes under agreement from Pfizer, Inc.