ORIGINThe transgene was constructed by inserting four copies of the chicken chromatin insulator sequence into the multiple cloning region of the pHelix1. An expression cassette fragment consisting of the neuron-specific enolase (NSE) promoter, a random filler sequence, and the SV40 polyadenylation signal sequence was then subcloned into a blunted Bam H1site on the pHelix/CI vector such that it was flanked on each side by two copies of the chromatin insulators. The G100L MMP-9 cDNA was subcloned as a Xba1fragment into the NHe1 and SPe1sites of the vector, replacing some of the random filler sequence. The transgene was purified using Elutip columns and used for pronuclear injection into FVB/N embryos, which were then transferred to pseudopregnant CD-1 females. Founder transgenics were identified by PCR using tail tissue. Founder mice were bred to FVB/N mates and transgene-positive offspring were used to maintain the individual transgenic lines. Transferred to Charles River under exclusive license in 2011.
STRAIN CODEComing Soon
MUTATION INFORMATIONMatrix Metalloproteinases (MMPs) are expressed and secreted from cells as inactive zymogens, the latency of which are maintained through a cysteine switch mechanism in the highly conserved region of the propeptide. Due to the tight regulation of enzyme activity, through a two-step process, transgenic animals overexpressing MMP-9 may give rise to increased expression of the latent enzyme, but not the active enzyme. By designing mutant forms of the enzyme that autocatalytically removed the prodomain to yield active enzyme, the effects of MMP-9 in the CNS can be studied. Multiple mutations were made by site-directed mutagenesis and several mutations disrupted the function of the cysteine switch mechanism, leading to dysregulation of MMP-9.
RESEARCH APPLICATIONInflammation, extracellular remodeling
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DISCLAIMERMMP9 mice may be subject to the licensed patents and are sold only for research purposes under agreement from Pfizer, Inc.
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Selecting the appropriate animal model for your studies is critical to the success of your research. This is why, in 2011, our Model Evaluation Program was created to offer researchers a risk-free opportunity to evaluate the quality of our animal models prior to allocating valuable budget money or study resources.
Now, to further support your research needs, we have expanded our program to include the option to evaluate both surgically altered models and rodent biospecimen products
Charles River offers animal models with combined surgeries or services (e.g., feeding studies with tissue collection) to help meet the dynamic needs of our customers.
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Visit us in booth #091 to learn more about our recent innovations including expanded diagnostic capabilities, a new online portal connecting clients with their colonies and a deep-rooted commitment to humane care.
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The International Bone and Mineral Society (IBMS) and the Japanese Society for Bone and Mineral Research (JSBMR) are excited to sponsor the second joint scientific meeting by the two organizations.
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This full-day seminar encompasses selected topics from our Charles River Short Course on Laboratory Animal Science. We would like to acknowledge Michigan State University for hosting this event and extend our heartfelt thanks to guest speaker Dr. Danielle Ferguson.
This position is responsible for serving as a as a study director (SD), project scientist (PS) and/or principal investigator (PI) in the direction and execution of assigned studies in compliance with GLP regulations as they apply to the conduct of nonclinical research; coordinating all phases of the study planning process with appropriate departments; generating high-quality project plans, protocols, amendments and reports appropriate for assigned studies; reviewing, interpreting, integrating and presenting data on assigned studies; and functioning as contact for the planning and execution of sponsor interaction related to assigned studies, including proposal management and study scheduling, conduct and reporting.
Charles River Laboratories, Inc.