research animal models

ORIGIN
NSE-p25 mice were created by pronuclear injection of an NSE-p25 transgene, produced internally at Pfizer, with transplantation of embryos to pseudopregnant CD-1 females. At weaning genomic DNA was isolated from tail tissue and founder transgenics were identified by PCR that specifically amplified DNA sequences from the human p25 cDNA. Founder mice were bred to FVB/N mates and transgenic offspring were used to maintain the transgenic line. Transferred to Charles River in 2011.

STRAIN CODE
Coming Soon

COAT COLOR
White (Albino)

MUTATION INFORMATION
NSE-p25 mice overexpress human p25, a known activator of cdk5, which is a protein kinase associated with hyperphosphorylation of microtubule proteins such as tau and neurofilament. Immunohistochemical assessment using Bielschowsky staining indicates the presence of silver-positive neurons and changes in the neuronal cytoskeletal organization are apparent at the ultrastructural level of primarily the amygdale, thalamus/hypothalamus and cortex. This increased cdk5 activity is sufficient to produce hyperphosphorylation of tau and neurofilament reminiscent of Alzheimer’s disease and other neurodegenerative disorders. Accordingly, the p25 mice display increased spontaneous locomotor activity and differences from control in the elevated plus-maze test.

RESEARCH APPLICATION
Alzheimer's disease

ADDITIONAL INFORMATION
For more information about NSE-p25 mice, please contact us at info@eu.crl.com.

DISCLAIMER
NSE-p25 mice may be subject to the licensed patents and are sold only for research purposes under agreement from Pfizer, Inc.