Overview Technical Resources OriginLCAT mice were developed by injecting a 7.6 kb DNA fragment containing the entire human LCAT (lecithin-cholesterol acyltransferase) gene, 550 bp of a 3’ untranslated region and the murine albumin promoter and enhancer sequences into pronuclei of 1-day-old superovulated female C57BL/6 embryos. Founders were identified by PCR amplification and confirmed by Southern blot using a full-length cDNA probe. Founder mice were bred to C57BL/6 mates to identify germline transmission and transgenic offspring were used to maintain the transgenic line. Transferred to Charles River under exclusive license in 2011. Coat ColorBlack Ideal For:Cholesterol efflux, esterification and transport Strain Code:522 HE, 523 HO, 524 WT, This model is cryopreserved. Paper: Expression of human lecithin:cholesterol acyltransferase in transgenic mice: effects on cholesterol efflux, esterification, and transport Genetically Engineered Model Services Dedicated Supply Backcrossing Rapid Colony Expansion
OriginLCAT mice were developed by injecting a 7.6 kb DNA fragment containing the entire human LCAT (lecithin-cholesterol acyltransferase) gene, 550 bp of a 3’ untranslated region and the murine albumin promoter and enhancer sequences into pronuclei of 1-day-old superovulated female C57BL/6 embryos. Founders were identified by PCR amplification and confirmed by Southern blot using a full-length cDNA probe. Founder mice were bred to C57BL/6 mates to identify germline transmission and transgenic offspring were used to maintain the transgenic line. Transferred to Charles River under exclusive license in 2011. Coat ColorBlack Ideal For:Cholesterol efflux, esterification and transport Strain Code:522 HE, 523 HO, 524 WT, This model is cryopreserved.
Paper: Expression of human lecithin:cholesterol acyltransferase in transgenic mice: effects on cholesterol efflux, esterification, and transport Genetically Engineered Model Services Dedicated Supply Backcrossing Rapid Colony Expansion