Overview Technical Resources OriginThe transgene was constructed by inserting four copies of the chicken chromatin insulator sequence into the multiple cloning region of the pHelix1. An expression cassette fragment consisting of the neuron-specific enolase (NSE) promoter, a random filler sequence, and the SV40 polyadenylation signal sequence was then subcloned into a blunted Bam H1site on the pHelix/CI vector such that it was flanked on each side by two copies of the chromatin insulators. The G100L MMP-9 cDNA was subcloned as a Xba1fragment into the NHe1 and SPe1sites of the vector, replacing some of the random filler sequence. The transgene was purified using Elutip columns and used for pronuclear injection into FVB/N embryos, which were then transferred to pseudopregnant CD-1 females. Founder transgenics were identified by PCR using tail tissue. Founder mice were bred to FVB/N mates and transgene-positive offspring were used to maintain the individual transgenic lines. Transferred to Charles River under exclusive license in 2011. Coat ColorWhite (albino) Ideal For:Inflammation, extracellular remodeling Strain Code:This model is cryopreserved. For more information about MMP9 mice please contact us at askcharlesriver@crl.com.
OriginThe transgene was constructed by inserting four copies of the chicken chromatin insulator sequence into the multiple cloning region of the pHelix1. An expression cassette fragment consisting of the neuron-specific enolase (NSE) promoter, a random filler sequence, and the SV40 polyadenylation signal sequence was then subcloned into a blunted Bam H1site on the pHelix/CI vector such that it was flanked on each side by two copies of the chromatin insulators. The G100L MMP-9 cDNA was subcloned as a Xba1fragment into the NHe1 and SPe1sites of the vector, replacing some of the random filler sequence. The transgene was purified using Elutip columns and used for pronuclear injection into FVB/N embryos, which were then transferred to pseudopregnant CD-1 females. Founder transgenics were identified by PCR using tail tissue. Founder mice were bred to FVB/N mates and transgene-positive offspring were used to maintain the individual transgenic lines. Transferred to Charles River under exclusive license in 2011. Coat ColorWhite (albino) Ideal For:Inflammation, extracellular remodeling Strain Code:This model is cryopreserved.