Overview Technical Resources OriginThe RIPHAT transgene consists of a PCR-generated cDNA encompassing the h-IAPP coding sequence under the regulation of the rat insulin II promoter/5’untranslated region and followed by intron I from the human albumin gene and the polyadenylation site/RNA termination region from the human glyceraldehydes-3-phosphate gene. Hemizygotes of the RHF line described in Couce et al. (Pfizer) were self-crossed to generate F1 offspring. Transgenic offspring were identified by PCR amplification of RIPHAT from tail tissue. Transferred to Charles River under exclusive license in 2011. Coat ColorWhite (albino) Ideal For:Diabetes Strain Code:534 HE, 535 HO, 536 WT, This model is cryopreserved. Paper: Spontaneous diabetes mellitus in transgenic mice expressing human islet amyloid polypeptide Genetically Engineered Model Services Dedicated Supply Backcrossing Rapid Colony Expansion
OriginThe RIPHAT transgene consists of a PCR-generated cDNA encompassing the h-IAPP coding sequence under the regulation of the rat insulin II promoter/5’untranslated region and followed by intron I from the human albumin gene and the polyadenylation site/RNA termination region from the human glyceraldehydes-3-phosphate gene. Hemizygotes of the RHF line described in Couce et al. (Pfizer) were self-crossed to generate F1 offspring. Transgenic offspring were identified by PCR amplification of RIPHAT from tail tissue. Transferred to Charles River under exclusive license in 2011. Coat ColorWhite (albino) Ideal For:Diabetes Strain Code:534 HE, 535 HO, 536 WT, This model is cryopreserved.
Paper: Spontaneous diabetes mellitus in transgenic mice expressing human islet amyloid polypeptide Genetically Engineered Model Services Dedicated Supply Backcrossing Rapid Colony Expansion