Expression Testing

Expression profiling data has proven useful in safety lead optimization and dose-limited toxicity studies, yielding insights sometimes not gleaned from traditional methods. Charles River offers low- and mid-density mouse and rat gene expression testing using pre-validated TaqMan® gene expression assays from Life Technologies.

Samples are tested in duplicate along with the endogenous control (18sRNA). ∆Ct values are calculated from the average target Ct values and the average endogenous Ct values. ∆∆Ct values are derived by using a known untreated or control for respective genes. Final relative quantitation (RQ) values are derived from ∆∆Ct values using the formula 2^-∆∆Ct. The RQ values represent the up or down regulation of gene expression in the sample relative to the control. 

  • Quantify the effect of RNA-mediated gene knockdown
  • Confirm data for individual or multiple genes
  • Measure steady-state level of mRNA expression from a transgene or knockout construct

Biomarker Services

Biomarker assays and readouts are important tools in drug discovery from target validation studies to candidate selection.

General Toxicology

Charles River performs general toxicology safety evaluation studies for the pharmaceutical, chemical, agrochemical and consumer product industries.