Cryopreservation & Cryorecovery Services

Embryo and sperm cryopreservation provides an effective and low-cost way to backup your live animal colony or store lines that are not actively being used. With over 20 years of experience and the knowledge gained from cryopreserving over 10,000 unique genetically engineered lines, our expertise helps ensure that your backup will be there when you need it. Stored embryos and sperm are divided between two independent locations, each with its own automated liquid nitrogen filling system and alarm network to help ensure the safety of your cryopreserved material.

Service Deliverables
Mouse Cryopreservation I 250-300 embryos collected utilizing 4-6 colony males and commercially available wild-type females
Mouse Cryopreservation II 150-200 homozygous embryos collected from colony males and females; requires 5-10 breeding pairs to initiate
Mouse Sperm Cryopreservation ~15 straws of sperm from 2 colony males
I•CRYO A do-it-yourself kit to cryopreserve and QC 5 lines via sperm
Rat Cryopreservation I 250-300 embryos collected utilizing 4-6 colony males and commercially available wild-type females
Rat Cryopreservation II 150-200 homozygous embryos collected from colony males and females; requires 5-10 breeding pairs to initiate
Rat Sperm Cryopreservation  ~15 straws of sperm from 2 colony males

Service Deliverables
Mouse Embryo Reconstitution 10 offspring if recovered from stock cryopreserved and stored at Charles River. Stock recovered from outside institutions will be evaluated for quality, and the number of animals recovered may vary.
Mouse Sperm Reconstitution 10 offspring if recovered from stock cryopreserved and stored at Charles River. Stock recovered from outside institutions will be evaluated for quality, and the number of animals recovered may vary.
Mouse Rapid Expansion Recovery numbers are variable, based on available stock.

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Cell Line Banking

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On-site Support

Charles River offers training and staffing to help manage animal facilities.