Please choose a project type

• Breeding
• Quarantine
• Cryopreservation
• Rederivation
• Reconstitution
• Line Expansion
• Genotyping
• Other

Breeding RFQ

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Breeding Project
Project description:
Animal Details	Species: Strain/line: Zygosity: Gender: Age: Quantity: * Choose Mouse Rat Other * Choose Homozygous Heterozygous Wild type Other Male Female +2nd Mouse Rat Other Choose Homozygous Heterozygous Wild type Other Male Female +3rd Mouse Rat Other Choose Homozygous Heterozygous Wild type Other Male Female +4th Mouse Rat Other Choose Homozygous Heterozygous Wild type Other Male Female +5th Mouse Rat Other Choose Homozygous Heterozygous Wild type Other Male Female +6th Mouse Rat Other Choose Homozygous Heterozygous Wild type Other Male Female
Background Strain:
Number of cages to be sent:
Project paradigm:
What sex of animals do you need produced:	Either sex Male Female
What zygosity do you need produced:	HETHOWTOther
Age (weeks):	From:    To:
How frequently do you need animals shipped:	One time As available Weekly Monthly Quarterly Other
How many animals do you need shipped in each order:
Would you like ear or tail samples to be collected from animals for genotyping?:	YesNo
If "Yes" above, Genotyping details:	Collected from: Animals shipped to Charles River Animals born at Charles River Both What type: Ear Tail Either Would you like: Genotyping performed at Charles River Samples shipped to you
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Quarantine RFQ

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Quarantine Project
Project description:
Animal Details	Species: Strain/line: Zygosity: Gender: Age: Quantity: * Choose Mouse Rat Other * Choose Homozygous Heterozygous Wild type Other Male Female +2nd Mouse Rat Other Choose Homozygous Heterozygous Wild type Other Male Female +3rd Mouse Rat Other Choose Homozygous Heterozygous Wild type Other Male Female +4th Mouse Rat Other Choose Homozygous Heterozygous Wild type Other Male Female +5th Mouse Rat Other Choose Homozygous Heterozygous Wild type Other Male Female +6th Mouse Rat Other Choose Homozygous Heterozygous Wild type Other Male Female
Background Strain:
Number of cages to be sent:
Would you like to breed during quarantine?:	YesNo
If you answered Yes to breeding, describe your project paradigm:
Would you like ear or tail samples to be collected from animals for genotyping?:	YesNo
If "Yes" above, Genotyping details:	Collected from: Animals shipped to Charles River Animals born at Charles River Both What type: Ear Tail Either Would you like: Genotyping performed at Charles River Samples shipped to you
Please indicate what Health Testing program should be performed on your project colony:	GEMS Standard Protocol (Comprehensive Health Monitoring and Helicobacter Screening) - Includes MFIA assessment plus, gross pathology, bacteriology and parasitology Custom Quarantine Protocol - Please describe protocol in the comments below
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Cryopreservation RFQ

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Cryopreservation Project - Mouse embryo, rat embryo, or mouse sperm
Package options:	Embryo Package I - Cryo of 250-300 embryos using wild-type females. Embryo Package II - Cryo of 150-200 embryos using homozygous females, includes expansion breeding. Sperm - 15 straws, includes pre- and post-thaw sperm analysis. Other - please describe in the Comments field below
Animal Details	Species: Strain/line: Zygosity: Gender: Age: Quantity: * Choose Mouse Rat Other * Choose Homozygous Heterozygous Wild-type Other Male Female +2nd Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +3rd Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +4th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +5th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +6th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female
Background Strain:
Number of cages to be sent:
Storage:	At Charles River Laboratories At your location Split between locations
Project paradigm:
Package I additional questions	Mating scheme: Homozygous male x Wild-type female Heterozygous male x Wild-type female Other Strain of wild type females: Source: Charles River Laboratories Other
Comments:
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Rederivation RFQ

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Rederivation Project - Mouse embryo, rat embryo, or mouse sperm
Package options:	Mouse Embryo Rederivation I - Guarantee of two visibly pregnant females shipped, no health testing, original males are held until rederived offspring are born at client's facility. We require 4-6 proven breeder males and wild-type females of selected strains included or 10-15 young colony females may be utilized. Mouse Embryo Rederivation II - Guarantee of 10 offspring, complete health testing, original males and remaining females held until rederived offspring are weaned and health reports are available. We require 4-6 proven breeder males and wild-type females of selected strains included or 10-15 young colony females may be utilized. Mouse Embryo Rederivation III - Expansion of homozygous colony, guarantee of 10 offspring, complete health testing, original colony held until rederived offspring weaned and health reports available. We require a minimum of 5-10 breeding pairs. Mouse Sperm Rederivation I - Guarantee of two visibly pregnant females shipped, no health testing, remaining sperm frozen. We require a minimum of 2 breeder males. Mouse Sperm Rederivation II - Guarantee of 10 offspring, complete health testing, remaining sperm frozen. We require a minimum of 2 breeder males. Rat Rederivation I - Guarantee of 10 offspring, complete health testing, original males and remaining females held until rederived offspring are weaned and health reports are available. We require 4-6 proven breeder males. Rat Rederivation II - Expansion of homozygous colony, guarantee of 10 offspring, complete health testing, orignal colony held until rederived offspring weaned and health reports available. We require a minimum of 5-10 breeding pairs.
Animal Details	Species: Strain/line: Zygosity: Gender: Age: Quantity: * Choose Mouse Rat Other * Choose Homozygous Heterozygous Wild-type Other Male Female +2nd Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +3rd Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +4th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +5th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +6th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female
Background Strain:
Number of cages to be sent:
Mouse Embryo Rederivation Packages additional question	Cryoreserve: YesNo
Mouse Sperm Rederivation Packages additional questions	Strain of wild type females: Source: Charles River Laboratories Other Sperm storage: At Charles River Laboratories At your location Split between locations
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Reconstitution RFQ

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Reconstitution Project - Mouse embryo, rat embryo, or mouse sperm
Project Type:	Mouse embryo reconstitution Mouse sperm reconstitution Rat embryo reconstitution Other
Product Details	Species: Strain/line: Zygosity: Storage: Quantity: * Choose Mouse Rat Other * Choose Homozygous Heterozygous Wild-type Other * Choose Vials Straws Other +2nd Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Choose Vials Straws Other +3rd Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Choose Vials Straws Other +4th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Choose Vials Straws Other +5th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Choose Vials Straws Other +6th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Choose Vials Straws Other
Background Strain:
Comments:
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Line Expansion RFQ

Mouse Rapid Expansion

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Rapid Expansion Project
Available packages:	Expansion I - 40-80 animals produced, we require 2-3 proven breeder males Expansion II - 81-120 animals produced, we require 2-3 proven breeder males Expansion III - 121+ animals produced, we require 3-5 proven breeder males
Animal Details	Species: Strain/line: Zygosity: Gender: Age: Quantity: Mouse * Choose Homozygous Heterozygous Wild-type Other Male Mouse Choose Homozygous Heterozygous Wild-type Other Male Mouse Choose Homozygous Heterozygous Wild-type Other Male Mouse Choose Homozygous Heterozygous Wild-type Other Male Mouse Choose Homozygous Heterozygous Wild-type Other Male Mouse Choose Homozygous Heterozygous Wild-type Other Male
Background Strain:
Number of cages to be sent:
How many animals should result from this expansion:
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Genotyping RFQ

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Genotyping services
What services are you interested in (check all that apply):	PCR Genotyping MAX-BAXSM Background Strain Characterization Zygosity Testing Strain-Specific Genetic Verification Expression Testing Chromosomal Mapping Tg Copy Determination
Species:	MouseRatZebrafishOther
Total number of samples to be tested:
PCR Genotyping additional info
Number of genes tested per sample:
Line(s) name:
MAX-BAXSM additional info
Starting background strain(s):
Desired background strain:
Current generation (if known):
Chromosomal location of gene:
Will the samples require genotyping by Genetic Testing Services:	YesNo
If yes, is the protocol in place:	YesNo
Background Strain Characterization additional info
Expected strain(s):
Zygosity Testing additional info
Testing type:	Common Element Gene Specific
If Common Element, check applicable options:	CreLacZNeoGFPLuciferaseHygromycinOther
Strain-Specific Genetic Verification additional info
Testing type:	SCID Testing NOD Testing MHCll Haplotype Testing Immorto Testing Obesity Assays Retinal Degeneration Other
Obesity Assays subtypes:	lepr(fa) (Zucker)lepr(cp) (Koletsky)
Any final comments?
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Other RFQ

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Other Project
Project description:
Animal Details	Species: Strain/line: Zygosity: Gender: Age: Quantity: * Choose Mouse Rat Other * Choose Homozygous Heterozygous Wild-type Other Male Female +2nd Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +3rd Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +4th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +5th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female +6th Mouse Rat Other Choose Homozygous Heterozygous Wild-type Other Male Female
Please describe your project needs:
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