In Vitro Bioassays

Potency determination is necessary for regulatory submission and lot release of all biopharmaceutical products. Charles River offers in vitro bioassays to cover a range of products, including assays targeting the emerging biosimilars market.

  • Cell proliferation assays
    • Cell-based potency assays for EPO, PTH, G-CSF and GM-CSF
    • Antiviral cell-based assays for measuring the potency of interferons (IFN-α, IFN-β)
  • Assays for monoclonal antibodies
    • Mode of action (MOA) assays: ADCC, CDC, apoptosis
    • Binding assays
    • Potency assays
    • Competitive assays
    • Neutralization assays
  • Immunogenicity testing
    • Anti-drug antibody (ADA) assays: binding and neutralizing antibodies
    • T-cell proliferation assays
    • Multiplex cytokine analysis by flow cytometry

Assay Readouts

  • Absorbance
  • Fluorescence
  • Time-resolved fluorescence
  • Luminescence

Statisical Evaluations

  • Parallel line analysis
  • Four-parameter fit
  • Five-parameter fit
  • EC50 determination

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