Glycoprotein Characterization

Charles River offers a comprehensive panel of assays for the characterization of glycoproteins, including primary, secondary, tertiary, higher order structures, and protein binding (affinity) methods. Services we offer include:

Primary Structure

  • LC-MS/MS Peptide Mapping
    • Amino Acid Sequence Verification
    • Post Translational Modification Mapping
    • N- and O-Linked Glycosylation Mapping
    • Oxidation, Deamidation, Glycation Mapping
  • LC-MS Intact Molecular Weight and Antibody-Drug-Conjugate Analysis
  • N-Terminal Sequence for N-terminal truncations
  • Amino Acid Analysis and Extinction Coefficient Determination

Secondary Structure

  • LC-MS Disulfide Bridge Mapping
  • Circular Dichroism (CD)
  • Fourier Transform Infrared (FTIR)
  • Intrinsic Tryptophan Fluorescence (ITF)
  • Differential Scanning Calorimetry (DSC)

Isoform and Purity Analysis

  • Capillary Electrophoresis:
    • cIEF
    • CE-SDS
    • Zone- electrophoresis
    • Imaging capillary electrophoresis (ICE)
  • SDS PAGE, and IEF
  • LC-MS/MS Impurity and Degradant Characterization
  • HPLC Methods such as RP HPLC, IEX

Aggregation, Particle Size, and Higher- Order Structures

  • Size Exclusion HPLC UV/MALLS
  • Analytical Ultracentrifugation (AUC)
  • SDS-PAGE
  • Dynamic Light Scattering (DLS)

Activity

  • Protein Binding Affinity via Surface Plasmon Resonance (SPR)
  • ELISA for Clone Selection programs through cGMP lot release
  • Enzymatic / Colorimetric Assays
  • Cell-based assays

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Mass Spectrometry

Charles River's mass spectrometry laboratory helps clients with the structural characterization of the large molecule products.

Biologics Testing

Charles River is able to help with all of your biologics testing needs from sterility and mycoplasma testing to stability and lot release programs.

Potency Testing

Charles River is able to provide expert opinions and services for the development and validation of in vivo and in vitro bioassays.

Poster: Comprehensive N-glycan Characterization

This poster describes a strategy for uniting quantitative profiling with comprehensive N-glycan characterization for the detailed structural understanding of a biopharmaceutical product.