Reference Standard Characterization & Support

Charles River offers a comprehensive panel of services for the characterization of reference standards.

Reference Standard Characterization Panel

  • Identity
    • Intact mass – MS, LC-MS
    • Peptide mapping – LC-MS/MS
    • Western blot
    • Chromatography
    • N-terminal sequencing
  • Primary structure
    • LC-MS/MS
  • Post-translational modifications:
    • Disulfide mapping – LC-MS/MS
    • Glycan analysis (N- and O-linked)
    • Oxidation – LC-MS/MS
    • Deamidation – LC-MS/MS/Isoquant™
    • N-terminus integrity – LC-MS/MS, N-terminal sequencing
    • C-terminus integrity – LC-MS/MS, Carboxypeptidase sequencing
    • Other PTMs – LC-MS/MS
  • Purity
    • RP-HPLC
    • IEX
    • HIC
    • SDS-PAGE
    • CE-SDS
  • Charge distribution
    • IEF
    • cIEF
    • Zone electrophoresis
    • Imaging capillary elelctrophoresis (ICE)
  • Secondary structure
    • CD
    • FTIR
    • ITF
  • Tertiary structure
    • ITF/Extrinsic fluorescence
    • DSC
  • Aggregation
    • SEC
    • SEC-MALLS
    • AUC
    • DLS
  • Potency
  • Strength
    • AAA
    • Absorbance at 280nm
    • Extinction coefficient by AAA
    • Colorimetric – Bradford/Lowry
  • Physiochemical
    • Appearance
    • pH
    • Osmolality
  • Biosafety
  • Other impurities

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