Epithelial Short-Circuit Current Assays

Ussing chamber assays (UCA) measure ionic currents across polarized epithelial cell layers (maintained in culture—either primary cells or clonal cell lines) in drug discovery and toxicology evaluations. Chronic and acute test article modulation of transepithelial current, resistance and potential are quantitated.

  • Determine concentration-response relationships for CFTR modulators or inhibitors
  • Screen against the ΔF508-CFTR (associated with cystic fibrosis [CF]) in CF patient-derived bronchial epithelial (CFhBE) primary cultures
  • Screen against wild-type CFTR in NhBE cells from normal patients
  • Evaluate effects on epithelial sodium channel (ENaC), an important target in hypertension and CF in NhBE

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