DSS Model | TNBS Model | Poly I:C Model
A single intrarectal instillation of haptenizing molecule 2,4,6-trinitrobenzene sulfonic acid (TNBS) in vehicle ethanol is known to induce immunogenic reactions in the colon wall and generate colitis in mice that models the human condition. CD4+ T cells are suggested to play a key role in inducing colitis in this mouse model. The acute TNBS colitis model is usually induced by a single intrarectal administration of TNBS and then observed for 5-7 days.
Histopathologically, the TNBS model is characterized by more focal damage in the colon than the DSS colitis model. Presensitization may be used to mimic a delayed type hypersensitivity reaction. Our inflammation group has validated this model for use in testing efficacy of novel therapeutic compounds.
Chemically Induced Mouse Models of Intestinal Inflammation. Nat Protoc, 2007. 2(3):541-6.