Adenoviral vectors are used to introduce shRNA or cDNA into cells in order to switch genes of interest off (knock-down by RNAi) or on (overexpress). Charles River has collections of arrayed adenoviruses focusing on druggable targets, greatly increasing the chances of ultimately discovering clinically effective therapies.
Our adenoviral system has some distinct advantages over conventional transfection technologies and the use of lentiviral vectors:
Efficient delivery to a wider range of human primary cells
Suitable for both transient and long-term expression of the RNAi or overexpression phenotype, without genomic integration
Arrayed format for high-throughput readout
Cherry-pick genes of interest or screen entire library
In addition to our off-the-shelf shRNA and cDNA collections, we can generate viruses expressing constructs to either overexpress or knock-down your genes of interest.