GLP Ion Channel Assays & Dose Formulation Analysis

Conventional manual patch clamp measurements of the concentration-response relationship in ion channels stably expressed in mammalian cells provide IC50 values for IND-enabling, cardiac risk evaluation.

Concentration verification via chemical analysis of dosing formulations strengthens the accuracy and thoroughness of nonclinical cardiac safety studies. DFA is a requirement for GLP studies submitted in an IND dossier.


  • Required for IND submissions
  • Quantitative assessment of inhibitory potency
  • Physiological temperature standard

Additional GLP ion channel assays include Cav1.2 (ICa,L), Nav1.5 (INa), Kv1.5 (IKur), Kv4.3 (ITO), KvLQT1/minK (IKs).

Dose Formulation Analysis (DFA)

  • Same-day DFA and hERG analysis
  • HPLC with UV detection

You may also be interested in...

high-throughput screening (HTS)

Discovery Ion Channel Assays

Charles River provides ion channel cell lines that are validated for testing at each stage of the drug discovery process.

Safety Pharmacology

Established ICH core battery tests and supplementary safety pharmacology models to meet current regulatory requirements and provide data to accurately drive your decision-making process.

IND-Enabling Studies

Learn how we can help you successfully initiate and complete your IND program.