LPS-Induced Uveitis Model

The lipopolysaccharide (LPS)-induced model of anterior uveitis allows for the evaluation of ocular anti-inflammatory treatments. Ocular inflammation can be caused by infection, allergy, trauma or reaction to drugs administered onto or into the eye.

For this assay, E. coli-derived LPS is injected into the vitreous humor of New Zealand White rabbits. This produces an acute transient inflammatory response that resolves spontaneously within approximately 72 hours. During this time period, the eyes can be evaluated by slit lamp ophthalmological examination. Aqueous humor can be tapped and evaluated for the amount of inflammatory cells present and the level of cytokines. Additionally, anti-inflammatory drug candidates can be screened against vehicle formulations to determine relative efficacy.

Primary Endpoints

  • Slit lamp ophthalmology examination
  • Aqueous humor (AH) cell counts
  • All endpoints completed by 72 hours post LPS
Treatment AH Cell Count Average AH Cell Count Range
None 1.0 cells/uL 0 - 4 cells/uL
LPS + Topical Saline 71 cells/uL 5 - 390 cells/uL
LPS + Topical Corticosteroid 31.5 cells/uL 2 - 57 cells/uL

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