Model of Retinal Degeneration & Neuroprotection

Light exposure is a common method of modeling the photoreceptor degeneration seen in human diseases such as retinitis pigmentosa and age-related macular degeneration. Charles River offers a blue light exposure model that induces retinal damage and cell death to be used as a screen for investigating potential neuroprotective properties of new chemical or biological entities (NCEs/NBEs) prior to subsequent nonclinical testing. With shorter study durations and the ability to ensure relatively uniform exposure and model optimization, this model is a favorable alternative to a genetically engineered model.

Model: Albino Mouse (BALB/c)
Endpoint: Outer nuclear layer thickness measured by:

  • Pathology
  • Optical coherence tomography (OCT)

Electroretinogram (ERG)

Intravitreal Treatment (IVT) Duration of Light Exposure (hours) ONL Thickness (cell layers) ERG Amplitude % change
Saline 6 2.24 >-90%
BDNF 6 11.53 +21%
Saline 24 1.24 >-90%
BDNF 24 10.78 - 42%
Baseline n/a 12 0
*460-490nm, 1100 lux

OCT Images

  • Eye A = baseline ONL
  • Eyes B-E = saline IVT
  • Eyes F-I = BDNF IVT
  • Green bar = ONL thickness

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Ocular Webinar

This webinar provides an overview of our ocular pharmacology models and safety assessment experience.