AccuGENX-ST™

As a complement to our sequence-based microbial identification service (AccuGENX-ID®), Charles River offers strain typing by single or multilocus sequence typing (SLST or MLST), both of which are highly accurate, sequence-based strain typing methods. We perform DNA sequencing of targets outside of the standard regions sequenced for microbial identification (16S, D2 or ITS2) to provide you with data to resolve differences and/or similarities at the strain level. Determination to the strain level allows you to definitively track the source of contamination or simply to verify your known production strains.

Given the reproducibility of these methods, they can determine if your probiotic strain is the same, or if isolates recovered from one area are the same or different as another isolate – a trait that allows for high-resolution trending and tracking projects.

Currently, we have established cGMP processes for specific species. The number and identity of the gene targets vary for each species due to the level of relatedness and rate of evolution within each group. The number ranges from 1-3 targets and, along with turnaround time, determines the cost of the strain typing service. To inquire about a species of interest available as a cGMP process, contact our Technical Support Services. If your organism is not on the list, inquire to find out what organisms are under R&D and when they will be available.

  • The foundation is built upon DNA sequencing results.
  • SLST/MLST data serve as primary resources for tracking and documenting the genetic relationship of bacteria at a global scale.
  • Results are easily cataloged and referenced for future comparisons.
  • These techniques are highly reproducible, unambiguous and scalable.

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AccuGENX-ST™

Strain typing using single and multi-locus sequence typing (SLST/MLST).