As a complement to our sequence-based microbial identification service (AccuGENX-ID®), we offer strain typing by single- or multi-locus sequence typing (SLST or MLST), both of which are highly accurate, sequence-based strain typing methods. We perform DNA sequencing of targets outside of the standard regions sequenced for microbial identification (16S, D2 or ITS2) to provide you with data to resolve differences and/or similarities at the strain level. Determination to the strain level allows you to definitively track the source of contamination or simply verify your known production strains.

Given the increased reproducibility of these methods, they can help determine if your probiotic strains are the same or if the isolates recovered from one area are the same or different as another isolate—an important trait that allows for high resolution and greater confidence in your trending and tracking projects than methods utilizing fragment-based analysis.

In order to achieve a higher degree of resolution, each species is researched by our R&D team to determine the gene targets outside of the region used for identification that will most definitively resolve them at the subspecies or strain level. The number and identity of these loci vary for each species, due to the level of relatedness and rate of evolution within each group. To inquire about a species of interest, contact our Technical Support Services.

  • The foundation is built upon DNA sequencing results.
  • SLST/MLST data serve as primary resources for tracking and documenting the genetic relationship of bacteria at a global scale.
  • Results are easily cataloged and referenced for future comparisons.
  • These techniques are highly reproducible, unambiguous and scalable.

You may also be interested in...

AccuGENX-ST® Technical Sheet

AccuGENX-ST® is used to distinguish microorganisms down to the strain level by utilizing methods based on single- and multilocus sequence typing (S/MLST).

Media plates for bacterial identification

Bacterial Sequencing (BacSeq)

AccuGENX-ID® utilizes comparative DNA sequencing of the 16S rRNA gene to produce reliable results in as little as six hours.

Highly Accurate Strain Analysis Through Sequence Typing

Highly Accurate Strain Analysis Through Sequence Typing

By combining standard genotypic identification methods with multilocus sequence typing (MLST) or singlelocus sequence typing (SLST), it is now possible to distinguish some of the most difficult organisms commonly observed in manufacturing industries for trending and tracking purposes.