Molecular Biology

Charles River performs a comprehensive array of genomics-based assays to support all phases of drug development, from discovery and preclinical studies through clinical trials, in either singleplex or multiplex formats. Our molecular biology facilities have custom-designed, dedicated laboratories with separate sample processing (nucleic acid extraction), reagent preparation and amplification/product detection areas to minimize the possibility of cross-contamination.

We isolate and purify DNA and RNA from a variety of tissues, body fluids and cells in culture. Our extraction procedures are optimized to provide the highest quality real-time qPCR results; both quality and yield of extracted nucleic acid samples are assessed prior to being used.

  • Nucleic acid therapeutic assessments
  • Gene expression analysis
  • Cytokine mRNA profiling in immune response
  • Biomarker assessment
  • Real-time quantitative and reverse transcriptase PCR
  • Reporter gene assays
  • Genomic and plasmid DNA isolation
  • Residual mammalian DNA detection by hybridization analysis
  • Assay feasibility, development and validation
  • Biological sample analysis
  • GLP/non-GLP study designs and reporting
  • Real-time PCR platforms capable of supporting multiple fluorescent chemistries
  • Luminex®-based QuantiGene® system
  • Bioluminescent reporter gene assays
  • Allelic discrimination assays (TaqMan® probe chemistry)

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Webinar Replay

Log in to the Source℠ to view our webinar that discusses preclinical biodistribution study designs, validation of real-time qPCR methods and procedural controls for sample analysis in a regulatory environment.

Biodistribution Study

Biodistribution studies are necessary to provide preclinical safety evaluation of novel gene therapy technologies or DNA vaccines.