In studies of RNA abundance and gene expression, no one technique can provide all of the answers so it is necessary to use a combination of several methods. Charles River has extensive experience working with immunohistochemical (IHC) procedures as well as in situ hybridization (ISH) techniques for localizing gene expression, proteins and mRNA.
When we pair our ISH methods with cell-type-specific immunostaining, we can generate a more complete picture of mRNA and protein expression within specific cell types in tissue sections. These assays can be used to assess tissue responses to various drug treatments for proof-of-principle and/or safety and efficacy evaluations.
We offer a range of validated IHC procedures and can develop new ones to answer study-specific questions. We also have a variety of both isotopic and non-isotopic ISH probes for a wide range of mRNAs, and new probes can be developed as needed.