Immunohistochemisty & Tissue Cross-Reactivity

Charles River has extensive experience working with immunohistochemistry procedures, as well as with a broad range of tissue-based biomarkers. In addition, we can develop new procedures to assist you in answering specific questions regarding the activity of your test materials. Interpretation of specimens can be performed subjectively by a pathologist, or advanced quantifiable techniques can be applied.

Among our experience and offerings:

  • Preclinical safety assessment for approximately 50-60 humanized, chimeric or murine monoclonal antibodies annually
  • Experience with unconjugated antibodies, antibodies conjugated to a variety of substances and Fab fragments
  • Techniques to reduce or eliminate the binding of secondary reagents to endogenous immunoglobulin to allow efficient and specific detection of bound unconjugated IgGs, particularly IgG1, even when the tissue is of the same species as the immunoglobulin

Charles River can support your monoclonal antibody research with our extensive expertise in the initial stages of safety assessment for therapeutic antibodies targeted for use in humans. We hold a full range of tissues from various animal species, including human tissues.

For immunohistochemistry-based tissue cross-reactivity studies, we provide efficiency of scale in full compliance with Good Laboratory Practice (GLP) regulations and can represent you in presenting and interpreting findings to the applicable regulatory agencies.

We provide correlative studies that are designed as an adjunct to clinical trials. These studies assist in determining whether a drug is reaching the intended target and whether the drug will work the same way in humans as it has in animals. Because each project has its own unique requirements, our pathologists and scientists will work closely with you to identify which tissue-based biomarkers to assess.

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