Dr. Masakazu Tsuchiya is a Senior Research Scientist for the Microbial Solutions division of Charles River. With more than 30 years experience in the detection of endotoxin and other microbial cell wall components, notable achievements include the development of tools for microbial cell wall components, such as an endotoxin-specific Limulus Amebocyte Lysate (LAL) reagent, a beta-glucan-specific LAL reagent, and the Silkworm Larvae Plasma (SLP) reagent to detect peptidoglycan and beta-glucan.
- International Journal of Development Research: Innovative Mechanism of Limulus Amebocyte Lysate Activation to Achieve Specificity and Sensitivity to Endotoxin; Comparison with Recombinant Factor C Reagents
- PDA Journal of Pharmaceutical Science and Technology: Sample Treatments That Solve Low Endotoxin Recovery Issues
- PDA Journal of Pharmaceutical Science and Technology: Factors Affecting Reduction of Reference Endotoxin Standard Activity Caused by Chelating Agent/Detergent Matrices: Kinetic Analysis of Low Endotoxin Recovery
- Immunome Research Journal: Mechanism of Low Endotoxin Recovery Caused by a Solution Containing a Chelating Agent and a Detergent
- American Pharmaceutical Review: Possible Mechanism of Low Endotoxin Recovery
1. Tsuchiya, M.: Decrease of endotoxin activity in solutions containing a chelating agent and a detergent (Mechanism of Low Endotoxin Recovery) (in Japanese). In Endotoxin Research vol. 21 (Ed: Japanese Endotoxin and Innate Immunity Society), Igaku Tosho Shuppan, Tokyo, p 23-25 (2018).
2. Tsuchiya, M.: Factors affecting Low Endotoxin Recovery (in Japanese). In Endotoxin Research vol. 20 (Ed: Japanese Endotoxin and Innate Immunity Society), Igaku Tosho Shuppan, Tokyo, p 19-21 (2017).
3. Tsuchiya, M.: Morphology change of lipopolysaccharide in Low Endotoxin Recovery (in Japanese). In Endotoxin Research vol. 19 (Ed: Japanese Endotoxin and Innate Immunity Society), Igaku Tosho Shuppan, Tokyo, p 19-23 (2016).
4. Tsuchiya, M.: Aspects of Low Endotoxin Recovery (LER) in US and Europe (in Japanese). In Endotoxin Research vol. 18 (Ed: Japanese Endotoxin and Innate Immunity Society), Igaku Tosho Shuppan, Tokyo, p 33-38 (2015).
5. Tsuchiya, M.: Bacterial Endotoxins Test –Second Edition- (in Japanese, whole book), Joho-kiko, Tokyo (2015).
6. Tsuchiya, M.: Reconfirmation of prerequisite condition for blood endotoxin measurement (in Japanese). In Endotoxin Research vol. 17 (Ed: Japanese Endotoxin and Innate Immunity Society), Igaku Tosho Shuppan, Tokyo, p 17-23 (2014).
7. Tsuchiya, M.: Harmonized Bacterial Endotoxins Test (in Japanese). In Biible of GMP/Validation (Ed: Kojima, H..), Gijutsu Joho Kyokai, Tokyo, p 351-355 (2012).
8. Tsuchiya, M.: Bacterial Endotoxins Test. In Microbiology and Sterility Assurance in Pharmaceuticals and Medical Devices (Ed: Saghee, M. R., Sandle, T., and Tidswell, E. C.), Business Horizons, New Delhi, p 363-428 (2011).
9. Tsuchiya, M.: The points and assay techniques of the Bacterial Endotoxins Test (in Japanese). In Know-how collection for quality testing and prevention of complaints/problems of products (Ed: Koda, R.), Gijutsu Joho Kyokai, Tokyo, p 293-307 (2008).
10. Tsuchiya, M.: Current aspect of plasma -glucan measurement by using kineticturbidimetric assay (in Japanese). In Useful clinical diagnostic methods for deep mycosis (Ed: Naoe, S.), Medical Sense, Tokyo, p 36-45 (2002).
11. Tsuchiya, M.: Endotoxin measurement (in Japanese). In Hemodialysis and Endotoxin (Ed: Takesawa, S.), Tokyo Igakusha, Tokyo, p 9-20 (2002).
12. Tsuchiya, M.: Current aspect of plasma Endotoxin measurement by using kinetic turbidimetric assay (in Japanese). In Endotoxin Research vol. 5 (Ed: Japanese Endotoxin Society), Igaku Tosho Shuppan, Tokyo, p 17-24 (2002).
13. Fujita, N., Ichiyama, Y., Horie, N., Sugihara, M., Hirahara, K., Murakami, N., Nomura, T. and Tsuchiya, M.: Reevaluation of plasma Endotoxin concentration in septic patients (in Japanese). In Endotoxin Research vol. 2 (Ed: Jpanese Endotoxin Society), Saikon Shuppan, Tokyo, p 49-57 (1999).
14. Suzuki, T., Katsuramaki, T., Furukawa, H., Kishida A., Harada, K., Tsuchiya, M. Matsuura, S. and Todo, S: Reevaluation of endotoxemia in liver transplant (in Japanese). In Endotoxin Research vol. 1 (Ed: Japanese Endotoxin Society), Saikon Shuppan, Tokyo, p 211-215 (1998).
15. Tsuchida, K., Takemoto, Y., Nakamura, T., Yamagami, S., Kishimoto, T. and Tsuchiya, M.: Meanings of measurement of plasma pyrogens and therapy in systematic inflammatory response syndrome (in Japanese). In Endotoxin Research vol. 1 (Ed: Japanese Endotoxin Society), Saikon Shuppan, Tokyo, p 205-210 (1998).
16. Tsuchiya, M.: Interfering factors on Endotoxin measurement (in Japanese). In Endotoxin (Eds: Nakano, M. and Kodama, M.), Kodansha Scientific, Tokyo, Chapter 7.5 (pp. 169-172) (1995).
17. Tsuchiya, M.: Another villain other than endotoxin (in Japanese). In Understanding Endotoxin in dialysate (Ed: Takesawa, S.), Tokyo Igakusha, Tokyo, pp. 135-148 (1995).
18. Harada, K., Tsuchiya, M., Matsuura, S., Inada, K., Yamashita, N. and Yoshida, M.: Evaluation of detergent dilution and heating method for plasma endotoxin measurement by using kinetic turbidimetric assay (in Japanese). In Recent evolution in clinical endotoxin research, Chugai Medical Information, Tokyo, p 17-24 (1993).
Journal Articles:
1. Dubczak, J., Reid, N., Tsuchiya, M.: Evaluation of limulus amebocyte lysate and recombinant endotoxin alternative assays for an assessment of endotoxin detection specificity. Eur. J. Pharm. Sci., 159, 105716 (2021). Doi: 10.1016/j.ejps.2021.105716.
2. Tsuchiya, M., Dubczak, J., Reid, N.: Beta-glucans in purified water – do they really cause overestimation of endotoxin values? Eur. Pharm. Rev., Application Notes & Whitepapers 2020, 12-13 (2020).
3. Otsuka, M., Nishi, Y., Tsukano, K., Tsuchiya, M., Lakritz, J., and Suzuki, K.: Sequential changes in hepatic mRNA abundance and serum concentration of serum amyloid A in cattle with acute inflammation caused by endotoxin. J. Vet. Med. Sci., 82, 1006-1011 (2020).
4. Nishi, Y., Tsukano, K., Otsuka, M., Tsuchiya, M., and Suzuki, K.: Relationship between bronchoalveolar lavage fluid and plasma endotoxin activity in calves with bronchopneumonia. J. Vet. Med. Sci., 81, 1043-1046 (2019).
5. Miyagawa, Y., Kikuchi, K., Tsuchiya, M., and Adachi, S.: A statistical model for activation of Factor C by binding to LPS aggregates. Eur. Biophys. J., 48, 743-747 (2019).
6. Tsuchiya, M.: Mechanism of Low Endotoxin Recovery caused by a solution containing a chelating agent and a detergent. Immunome Res., 15, 1-10 (2019).
7. Tsuchiya, M.: Sample treatment that solve Low Endotoxin Recovery Issues. PDA J. Pharm. Sci. Technol., 73, 433-442 (2019).
8. Tsuchiya, M.: Factors affecting reduction of reference endotoxin standard activity caused by chelating agent/detergent matrices: Kinetic analysis of Low Endotoxin Recovery. PDA J. Pharm. Sci. Technol., 71, 478-487 (2017).
9. Sotohira, Y., Suzuki, K., Otsuka, M., Tsuchiya, M., Shimamori, T., Nishi, Y., Tsukano, K., and Asakawa, M.: Plasma endotoxin activity in Eastern grey kangaroos (Macropus giganteus) with lumpy jaw disease. J. Vet. Med. Sci., 79, 1138-1141 (2017).
10. Suzuki, Y., Suzuki, K., Shimamori, T., Tsuchiya, M., Niehaus, A., and Lakritz, J.: Evaluation of portable test system for assessing endotoxin activity in raw milk. J. Vet. Med. Sci., 78, 49-53 (2016).
11. Sotohira, Y., Suzuki, K., Sasaki, H., Sano, T., Tsuchiya, M., Suzuki, Y., Shimamori, T., Tsukano, K., Sato, A., Yokota, H., and Asakawa, M.: Plasma endotoxin activity in kangaroos with oral necrobacillosis (lumpy jaw disease) using an automated handheld testing system. J. Vet. Med. Sci., 78, 971-976 (2016).
12. Suzuki, K., Shimamori, T., Sato, A., Tsukano, K., Tsuchiya, M., and Lakritz, J.: Detecting endotoxin activity in bovine serum using automated testing system. J. Vet. Med. Sci., 77, 977-979 (2015).
13. Miki, K., Hiratsuka, F., Suzuki, T., Tsuchiya, M., Oikawa, S., Nakada, K. and Tamura, Y.: Basic investigation on availability of portable endotoxin test system for the inspection of quarter and bulk milk from cows (in Japanese). Jpn. J. Animal Hyg., 40, 7-12 (2014).
14. Tsuchiya, M.: Aspects in the Bacterial Endotoxins Test and its analysis considering error factors (in Japanese), Bull. Osaka Phar. Manufactur. Assoc., 745, 64-98 (2011).
15. Tsuchiya, M.: Is the potency of your standard endotoxin correct? Eur. Pharm. Rev., 6, 29 (2010).
16. Tsuchiya, M: Biases in the Bacterial Endotoxins Test. BioProcess International industry Yearbook 2010, 2-3 (2010).
17. Tsuchiya, M: Advantages of archived standard curves for the quantitative Bacterial Endotoxins Test. BioProcess International, 7, 152-153 (2009).
18. Shibata, A., Ohwada, K., Tsuchiya, M. and Kogure, K.: Particulate peptidoglycan in seawater determined by the silkworm larvae plasma (SLP) assay. J. Oceanogr., 62, 91-97 (2006).
19. Shimizu, T., Endo, Y., Tabata, T., Mori, T., Hanasawa, K., Tsuchiya, M. and Tani, T.: Diagnostic and predictive value of the silkworm larvae plasma test for postoperative infection following gastrointestinal surgery. Crit. Care Med., 33, 1288-1295 (2005).
20. Ogikubo, Y., Norimatsu, M., Noda, K., Takahashi, J., Inotsume, M., Tsuchiya, M. and Tamura, Y: Evaluation of the bacterial endotoxin test for quantification of endotoxin contamination of porcine vaccines. Biologicals, 32, 88-93 (2004).
21. Inada, K., Takahashi, K., Ichinohe, S., Suda, H., Tsuchiya, M., Takahashi, J., Matsuura, S., Kasai, T., Yoshida, M., Endo, S. and Sato, S.: A silkworm larvae plasma test for detecting peptidoglycan in cerebrospinal fluid is useful for the diagnosis of bacterial meningitis. Microbiol. Immunol., 47, 701-707 (2003).
22. Endo, Y., Shimizu, T., Mori, T., Tabata, T., Hanasawa, K., Tsuchiya, M. and Tani, T.: Hyperoxic condition prevents bacterial translocation and elevation of plasma microorganism components during hemorrhagic shock. J. Investigative Sur., 16, 275-281 (2003).
23. Shimizu, T., Tani, T., Endo, Y., Tabata, T., Mori, T., Hanasawa, K., Tsuchiya, M. and Kodama, M.: Elevation of plasma peptidoglycan and peripheral blood neutrophil activation during hemorrhagic shock: Plasma peptidoglycan reflects bacterial translocation and may affect neutrophil activation. Crit. Care Med., 30, 77-82 (2002).
24. Tobita, T., Obayashi, K., Senpuku, Y., Tachibana, A., Hatakeyama, S., Suzuki, K., Umeya, T., Ichikawa, N., Ohi, S., Kodaira, M., Matsuura, H., Isogai, Y., Hishinuma, M., Shinozaki, S., Hayashi, C., Yagyu, T., Fumiiwa, H., Yamada, A., Yamashita, M., Imura, M., Mihara, T., Tanaka, Y., Ishihara, M., Tada, H., Tanaka, K. and Tsuchiya, M.: Benefits of a plasma endotoxin assay by supersensitive reagent for diagnosis of Gram-negative bacteremia (in Japanese with English abstract). Jpn. J. Crit. Care Endotoxemia, 5, 15-19 (2001).
25. Tsuchiya, M., Nishino, T., Ijiri, H. and Matsuura, S.: Performance of the Limulus amebocyte lysate for endotoxin detection in dialysate (in Japanese). Jin To Touseki, 47 (suppl.: HDF Treatment ’99), 61-64 (1999).
26. Fujiwara, H., Kira, K., Asakawa, S., Naito, S., Tanaka, S., Aizawa, M., Tsuchiya, M., Takaoka, A., Yamamoto, A., Ochiai, M. and Horiuchi, Y.: The application of the endotoxin testfor globulin and other blood products (in Japanese with English abstract). Yakugaku Zasshi, 119, 451-455 (1999).
27. Nakamura, R., Tazawa, S. and Tsuchiya, M.: The clinical significance of plasma (1-3)--D-glucan measurement by the kinetic turbidimetric Limulus test for fungal febrile episodes (in Japanese with English abstract), JARMAM, 9, 33-39 (1998).
28. Tsuchiya, M. and Takaoka, A.: Super sensitive endotoxin detection in dialysate (in Japanese). Jin To Touseki, 44 (suppl.: HDF Treatment ’98), 8-10 (1998).
29. Tsuchiya, M., Takaoka, A., Ijiri, H., Nishino, T., Murakami, N., Morita, M. Kanda, C. and Matsuura, S.: Endotoxin measurement in dialysate by using Endotoxin-Single Test Wako (in Japanese). Jin To Touseki, 43 (suppl.: HDF Treatment ’97), 145-149 (1997).
30. Tsuchida, K., Takemoto, Y., Yamagami, S., Edney, H., Niwa, M., Tsuchiya, M., Kishimoto, T. and Shaldon, S.: Detection of peptidoglycan and endotoxin in dialysate, using silkworm larvae plasma and Limulus amebocyte lysate methods, Nephron, 75, 438-443 (1997).
31. Tsuchida, K., Takemoto, Y., Fu, O., Okada, C., Kakitani, Y., Imamura, E., Kuboura, K., Arakawa, M., Yamagami, S., Kishimoto, T. and Tsuchiya, M.: Meanings of pyrogen measurement in extracorporeal circulation therapy (in Japanese). Jin To Touseki, 43 (suppl.: High Performance Membranes ’97), 103-108 (1997).
32. Tsuchida, K., Takemoto, Y., Yamagami, S., Kishimoto, T., Niwa, M. and Tsuchiya, M.: Peptidoglycan contamination of dialysate and its biological effects (in Japanese with English abstract). J. Jpn. Soc.Dialysis Ther., 30, 879-887 (1997).
33. Tsuchiya, M., Asahi, N., Suzuoki, F., Ashida, M. and Matsuura, S.: Detection of peptidoglycan and -glucan with silkworm larvae plasma test, FEMS Imunol. Med. Microbiol., 15, 129-134 (1996).
34. Tsuchiya, M., Ijiri, H., Takaoka, A. and Matsuura, S.: Sensitive measurements of endotoxins with the kinetic turbidimetric Limulus test (in Japanese with English abstract), J. Antibact. Antifung. Agents, 24, 179-183 (1996).
35. Tsuchiya, M., Asahi, N., Suzuoki, F. and Matsuura, S.: Detection of microbial contamination in sterile disposable wares with silkworm larvae plasma reagent (in Japanese with English abstract), J. Antibact. Antifung. Agents, 24, 257-261 (1996).
36. Tsuchiya, M., Inoue, K., Harada, K., Takaoka, A. and Matsuura, S.: Effective extraction of absorbed endotoxins with human serum albumin solution (in Japanese with English abstract), J. Antibact. Antifung. Agents, 24, 357-362 (1996).
37. Norimatsu, M., Ogikubo, Y., Aoki, A., Takahashi, T., Watanabe, G., Taya, K., Sasamoto, S., Tsuchiya, M. and Tamura, Y.: Effects of aluminum adjuvant on systemic reactions of lipopolysaccharides in swine, Vaccine, 13, 1325-1329 (1995).
38. Asakawa, S., Fujiwara, H., Naitoh, S., Honma, R., Ishida, S., Chino, F., Tsuchiya, M., Matsuura, S., Tanaka, S. and Ohki, M.: Application of the Limulus test for practical quality control on endotoxin content in commercial human serum albumin (HSA) products. In comparison with the rabbit pyrogen test (in Japanese with English abstract), Yakugaku Zasshi, 114, 888-893 (1994).
39. Takesawa, S., Hidai, H., Sugano, M. and Tsuchiya, M.: Fundamental study of measurement of endotoxin in dialysate (in Japanese with English abstruct). Jpn. J. Art. Organs, 23, 449-452 (1994).
40. Tsuchiya, M., Takaoka, A., Yamagata, Y., Matsuura, S., Hosobuchi, K. and Ishizeki, C.: Limulus amebocyte lysate test for medical devices (in Japanese with English abstract), J. Antibact. Antifung. Agents, 20, 139-145 (1992).
41. Naitoh, S., Fujiwara, H., Asakawa, S., Honma, R., Ishida, S., Chino, F., Tanaka, S., Ohki, M., Tsuchiya, M. and Matsuura, S.: Application of the Limulus amebocyte lysate test to measurement of endotoxin in therapeutic human plasma protein fraction. Comparison with the rabbit pyrogen test (in Japanese with English abstract), Yakugaku Zasshi, 112, 551-556 (1992).
42. Inada, K., Yamashita, N., Yoshida, M., Harada, K., Tsuchiya, M. and Matsuura, S.: Evaluation of detergent-dilution and heating method for measurement of endotoxin in plasma (in Japanese), Kiso To Rinsho, 26, 173-179 (1992).
43. Tani, T., Chang, T. M. S., Kodama, M. and Tsuchiya, M.: Endotoxin removed from hemoglobin solution using polymyxin-B immobilized fiber (PMX-F) followed by a new turbidimetric endotoxin assay, Biomat., Art. Cells & Immob. Biotech., 20, 457-462 (1992).
44. Kambayashi, J., Yokota, M., Sakon, M., Shiba, E., Kawasaki, T., Mori, T., Tsuchiya, M., Oishi, H.and Matsuura, S.: A novel endotoxin-specific assay by turbidimetry with Limulus amoebocyte lysate containing ß-glucan, J. Biochem. Biophys. Methods, 22, 93-100 (1991).
45. Tsuchiya, M., Oishi, H., Takaoka, A., Fusamoto, M. and Matsuura, S.: Discrimination between endotoxin and (1-3)--glucan using turbidimetric kinetic assay with Limulus amebocyte lysate, Chem. Pharm. Bull., 38, 2523-2526 (1990).
46. Tsuchiya, M., Takaoka, A., Tokioka, N. and Matsuura, S.: Development of an endotoxin-specific Limulus amebocyte lysate blocking ß-glucan-mediated pathway by carboxymethylated curdlan and its application (in Japanese with English abstract), Jpn. J. Bacteriol., 45, 903-911 (1990).
47. Fujiwara, H., Ishida, S., Shimazaki, Y., Naitoh, S., Tsuchiya, M. and Matsuura, S.: Measurement of endotoxin in blood products using an endotoxin-specific Limulus test reagent and its relation to pyrogenic activities in rabbit (in Japanese with English abstract), Yakugaku Zasshi, 110, 332-340 (1990).
48. Yokota, M., Kambayashi, J., Tsujinaka, T., Sakon, M., Mori, T., Tsuchiya, M., Oishi, H. and Matsuura, S.: A new method for the quantification of ß-glucan in plasma and its application in the diagnosis of postoperative infection, Jpn. J. Surgery, 20, 559-566 (1990).
49. Oishi, H., Fusamoto, M., Hatayama, Y., Tsuchiya, M., Takaoka, A. and Sakata, Y.: An automated analysis system of Limulus amebocyte lysate (LAL) - Endotoxin reaction kinetics using turbidimetric kinetic assay, Chem. Pharm. Bull., 36, 3012-3019 (1988).
50. Cannon, G. W., Tsuchiya, M., Rittschof, D. and Bonaventura, J.: Magnesium dependence of endotoxin-induced degranulation of Limulus amebocytes, Biol. Bull., 171, 330-337 (1986).
51. Taniguchi, M., Wakamiya, K., Tsuchiya, M., Matsuno, R. and Kamikubo, T.: Continuous Ethanol Production by Cell-Holding Culture of Yeast, Eur. J. Appl. Microbiol. Biotechnol., 18, 201-206 (1983).
52. Nakatani, H.,., Morita, T., Ohki, S. and Hiromi, K.: Binding of Streptomyces Pepsin Inhibitor (Acetyl-Pepstatin) with Chymosin (Rennin), Biochim. Biophys. Acta, 614, 144-150 (1980).
Reviews:
1. Tsuchiya, M.: Innovative mechanism of Limulus amebocyte lysate activation to achieve specificity and sensitivity to endotoxin; Comparison with recombinant Factor C reagents. Int. J. Dev. Res., 10, 36751-36756 (2020).
2. Tsuchiya, M.: Specificity of Limulus amebocyte lysate and recombinant reagents (in Japanese). J. Antibact. Antifung. Agents, 48, 205-208 (2020).
3. Tsuchiya, M.: Reconsideration on validation in Bacterial Endotoxins Test (in Japanese). J. Antibact. Antifung. Agents, 48, 11-15 (2020).
4. Tsuchiya, M.: Low Endotoxin Recovery (LER) (in Japanese). J. Antibact. Antifung. Agents, 47, 501-505 (2019).
5. Tsuchiya, M.: Possible mechanism of Low Endotoxin Recovery. Am Pahrm Rev, 17, 18-23 (2014).
6. Tsuchiya, M.: International aspects on the Bacterial Endotoxins Test (in Japanese). J. Antibact. Antifung. Agents, 39, 603-616 (2011).
7. Tsuchiya, M.: Endotoxin (in Japanese). J. Antibact. Antifung. Agents, 35, 383-389 (2007).
8. Tsuchida, K., Takemoto, Y., Yamagami, S., Kishimoto, T., Niwa, M. and Tsuchiya, M.: Peptidoglycan contamination in dialysate and its biological activity (in Japanese). J. Antibact. Antifung. Agents, 25, 405-412 (1997).
9. Ijiri, H. and Tsuchiya, M.: Inactivation of endotoxins by electron beam (in Japanese). Clean Technology, 6, 18-20 (1996)
10. Tsuchiya, M.: Development of a simple and sensitive detection system for components of microbial cell walls (in Japanese), J. Antibact. Antifung. Agents, 24, 593-600 (1996).
11. Tsuchiya, M.: Limulus test; a safety test for parenteral drugs such as biological products (in Japanese), BIO Clinica, 117, 74-78 (1995).
12. Ashida, M. and Tsuchiya, M.: Specific bio-defense reaction system in insects and its application (in Japanese). Brain Techno News, 31, 1-5 (1992).
13. Tsuchiya, M.: Limulus test and its application (in Japanese), J. Antibact. Antifung. Agents, 18, 287-294 (1990).
Patents:
1. Tsuchiya, M.: Heat-treated Limulus amebocyte lysates (2010)
- U. S. Patent No. 10473663 (2019)
- European Patent No. 2446018 (2017)
- Japanese Patent No. 5981338 (2016)
2. Ashida, M., Ochiai, M., Tsuchiya, M.: Peptidoglycan recognition protein and their production (1997)
- U. S. Patent No. 6034217 (2000)
- U. S. Patent No. 6413729 (2002)
3. Tsuchiya, M.: Process for detecting microorganisms (1995)
- U. S. Patent No. 5747277 (1998)
- European Patent No. 0757103 (2000)
4. Takaoka, A., Tsuchiya, M., Kawabe, K., Ijiri, H.: Process for measuring endotoxin or beta-1,3-glucan (1995)
- Japanese Patent No. 3327076 (2002)
- U. S. Patent No. 5637474 (1997)
- European Patent No. 0709681 (2002)
5. Ashida, M., Kawabata, T., Hirayasu, K., Tsuchiya, M.: Process for measuring prophenoloxidase activating enzyme activity (1994)
- Japanese Patent No. 3658782 (2005)
- U. S. Patent No. 5585248 (1996)
- European Patent No. 0657546 (2002)
- Taiwan Patent No. 088803 (1997)
6. Tsuchiya, M., Harada, K.: Process for inhibiting activity of endotoxin (1994)
- Japanese Patent No. 3106839 (2000)
- U. S. Patent No. 5616557 (1997)
- U. S. Patent No. 5622833 (1997)
- U. S. Patent No. 5750500 (1998)
- European Patent No. 0608982 (1997)
7. Tsuchiya, M., Takaoka, A., Harada, K.: Process for extracting endotoxin (1992)
- Japanese Patent No. 2884975 (1999)
- U. S. Patent No. 5346889 (1994)
- European Patent No. 0549239 (1997)
8. Tsuchiya, M., Harada, K.: Pretreatment of sample for endotoxin measurement (1992)
- Japanese Patent No. 2737514 (1998)
- U. S. Patent No. 5476772 (1995)
- European Patent No. 0552965 (1998)
9. Tsuchiya, M., Oishi, H., Takaoka, A., Matsuura, S.: Process for measuring endotoxin (1989)
- Japanese Patent No. 2090168 (1996)
- European Patent No. 0350273 (1994)
10. Tsuchiya, M., Matsuura, S.: Process for Preparing reagent for measuring endotoxin (1988)
- Japanese Patent No. 1984900 (1995)
- U. S. Patent No. 5047353 (1991)
- European Patent No. 0333187 (1993)
- Canadian Patent No. 1334276 (1995)
11. Tsuchiya, M., Matsuura S.: Process for measuring endotoxin (1988)
- Japanese Patent No. 2140974 (1999)
- U. S. Patent No. 5179006 (1993)
- U. S. Patent No. 5318893 (1994)
- European Patent No. 0330991 (1994)
- Canadian Patent No. 1339881 (1998)
12. Tsuchiya, M., Takaoka, A., Fusamoto, M.: Process for distinguishing measurement (between endotoxin and beta-glucan) (1988)
- Japanese Patent No. 2090148 (1996)
13. Ashida, M., Tsuchiya, M., Sakata, Y., Matsuura, S.: Reagents for determining peptidoglycan and beta-1,3-glucan (1986)
- Japanese Patent No. 2090107 (1996)
- Japanese Patent No. 2090108 (1996)
- U. S. Patent No. 4970152 (1990)
- European Patent No. 0270039 (1995)
14. Ashida, M., Tsuchiya, M., Sakata, Y., Matsuura, S.: Process for collecting body fluid from insects (1986)
- European Patent No. 0634656 (2000)