Robust Reagents. Rapid Results.

Traditional methods of microbial testing rely heavily on lengthy incubation periods to achieve visible growth, for colonies to grow large enough for confident enumeration in microbial limits, or for unambiguous turbidity in sterility testing. Celsis® ATP bioluminescence-based rapid detection can reduce these incubation periods by 50%.

As an automated rapid microbial test for aseptic and sterile manufacturing processes, Celsis® ATP-bioluminescence reagents will quickly screen for the absence or presence of bacteria, yeasts, and molds, allowing the release of final products through instrument-based analysis, following a shortened incubation time.

Celsis® ATP-bioluminescence Reaction Overview

Celsis Advance II instrument for rapid microbial detection

The fundamentals of Celsis® reagents are simple, yet powerful. Celsis® rapid microbial detection reagent technology is based upon a naturally occurring, ATP-bioluminescence reaction, which also provides the characteristic glow of fireflies.

Known as the luciferin-luciferase reaction, it occurs when the luciferase enzyme converts luciferin into oxyluciferin to produce light. Only when ATP is present will this reaction occur. Since ATP is present in all living cells, including prokaryotes, it can be used as a marker for contamination if a sample should not, under normal conditions, contain ATP.


Celsis AMPiScreen Amplified bioluminescence

Celsis AMPiScreen® reagents rapidly detect the presence of ultra-low levels of microbiological contamination up to 50% faster than Celsis LumiScreen™ reagents, with compatible sample types. The amplified-ATP assay, used in Celsis AMPiScreen®, is not constrained by the limited amount of metabolic ATP in the organism. Instead, it produces additional ATP by using an enzyme-catalyzed reaction and an additional instrument-controlled amplification period to optimize the detection of microorganisms. Learn More


Luciferase enzyme producing bioluminescence

Celsis LumiScreen reagents, utilizing the industry gold-standard ATP-bioluminescence detection method, combine the power of a confident presence/absence contamination detection with an impressive compatibility portfolio of sample types. If no microbial contamination is present, the reaction does not occur and no light is produced, regardless of difficult-to-filter, opaque, or viscous sample types. Learn More

Features of Celsis® Rapid Microbial Detection Reagents

  • Celsis LumiScreen™ and Celsis AMPiScreen® Reagents are compatible with Celsis Accel® and Celsis Advance II™ luminometers.
  • Optimized reagent kit sizes and volumes to minimize waste and improve reagent consumption efficiency.
  • Leverages the same in-house expertise that produces our industry-leading FDA-licensed Endosafe® reagents, trusted across the pharmaceutical industry.