Avian Testing Services

Charles River offers a wide array of avian diagnostic and regulatory testing services, including serological profiling, virus isolation and vaccine testing. Protocols are available for standard testing methods and customized services.

  • Multiplexed Fluorometric Immunoassay® (MFIA®) Tests
    MFIA® testing can only be performed on SPF and research flocks and is performed on individual chicken samples. Please provide 0.05 ml per sample.
    Test Description Test #
    14 Panel MFIA® Test
    (HEV, AE, ANV, PMV-2, Reo, F. Pox, IBV, IBDV, ALV-A, ALV-B, ALV-J, NDV, AI & ILT)
    15100327
    22 Panel MFIA® Test
    (14 panel and Adeno Grp I, Adeno Grp III, Rota, MD, Rev, MG, MS, & Salmonella Pullorum-Gallinarum)
    15100329
    Single Plex - Individual analytes as listed above Call for more info
  • Serum Plate Agglutination Tests
    Please provide 0.1 ml serum per test.
    Pathogen Test #
    Mycoplasma Gallisepticum 15100012
    Mycoplasma Synoviae 15100013
    Salmonella Pullorum-Gallinarum 15100016

     

  • HA-HI Assays
    Please provide 0.025 ml serum per hemagglutination (HA) and hemagglutination-inhibition (HI) test.
    Pathogen Test #
    Adenovirus Group III (EDS 76) 15100019
    Avian Influenza Type A 15100020
    Avian Paramyxovirus Type 2 15100029
    Avian Paramyxovirus Type 3 15100030
    Human Influenza A/PR/8/34 15100098
    Infectious Bronchitis (Ark.) 15100021
    Infectious Bronchitis (Conn.) 15100022
    Infectious Bronchitis (DE-072) 15100025
    Infectious Bronchitis (JMK) 15100023
    Infectious Bronchitis (Mass.) 15100024
    Mycoplasma Gallisepticum 15100026
    Mycoplasma Synoviae 15100027
    Newcastle Disease 15100028
  • Serum Neutralization Tests in SPF Eggs
    Please provide 2.0 ml serum per test.
    Pathogen Test #
    Avian Adenovirus (CELO-Phelps) 15100032
    Avian Encephalomyelitis 15100033
    Infectious Bronchitis (Ark.) 15100034
    Infectious Bronchitis (Conn.) 15100035
    Infectious Bronchitis (JMK) 15100036
    Infectious Bronchitis (Mass.) 15100037
    Infectious Bursal Disease (Gumboro) 15100038
    Infectious Bursal Disease (Variant E) 15100039
    Infectious Laryngotracheitis 15100040
    Newcastle Disease 15100041
  • Agar Gel Precipitin Tests
    Please provide 0.050 ml serum per test.
    Pathogen Test #
    Avian Adenovirus Group I 15100042
    Avian Adenovirus Group II (HEV) 15100048
    Avian Encephalomyelitis 15100043
    Avian Reovirus (Viral Arthritis) 15100045
    Avian Rotavirus 15100046
    Fowl Pox 15100047
    Infectious Bursal Disease (Gumboro) 15100050
    Infectious Bursal Disease Sero Type 2 15100052
    Infectious Laryngotracheitis 15100051
    Influenza 15100044
    Marek’s Disease 15100053
    Newcastle Disease 15100054
    Reticuloendotheliosis 15100055
  • Microneutralization Tests
    Please provide 0.25 ml serum per test.
    Pathogen Test #
    Avian Adenovirus (each sample tested individually for 12 serotypes) 15100056
    Avian Reovirus 15100057
    RSV-A 15100083
    RSV-B 15100084
    Avian Leukosis Subgroup J 15100085
  • IFA Testing
    Please provide 0.1 ml per serum sample. Chicken sera only.
    Pathogen Test #
    Chicken Anemia Virus 15100075
    Avian Nephritis Virus 15100078
    Avian Reovirus 15100080
    Reticuloendotheliosis Virus 15100081

     

  • 9 CFR Testing
    Please provide the quantities and volumes listed below.
    9CFR # Test Description Test #
    113.26 Detection of Viable Bacteria and Fungi Except in Live Vaccine* 15100071
    113.27 113.27 Detection of Extraneous Viable Bacteria and Fungi in Live Vaccine (10 x 0.5 ml) 15100070
    113.28 Detection of Mycoplasma Contamination (1.5 ml) 15100072
    113.30 Detection of Salmonella Contamination (2.5 ml of cells or 11 ml of virus) 15100073
    113.31 Detection of Avian Lymphoid Leukosis
    (A p27 ELISA is performed in place of CF; 5 ml of cells or 1 ml of virus with 1 ml of antiserum)
    15100059
    113.34 Detection of Hemagglutinating Viruses (2.5 ml) 15100067
    113.37 Detection of Pathogens by the Chicken Embryo Inoculation Test** 15100068
    113.46 Detection of Cytopathogenic and/or Hemadsorbing Agents (2.5 ml) 15100069
    * Each final product serial: 10 x 2.5 ml. Cells & cell lines and ingredients of animal origin: 1 x 22 ml.
    ** 5 ml of cells or 1 ml of virus with 10 ml of antiserum.
  • PCR Tests

    Gel-Based PCR

    Please provide 0.50 ml per sample.
    Pathogen Test #
    Chicken Anemia Virus 15100076
    Mycoplasma synoviae and gallisepticum 15100061
    Reticuloendotheliosis (REV) 15100062

    Endpoint & Real-time PCR

    Cloacal swabs or other samples upon request.
    Pathogen Test #
    Avian Adenovirus Group I, Serotypes 1–12 15100099
    Avian Adenovirus Group III (EDS) 15100309
    Avian Encephalomyelitis 15100101
    Avian Reovirus 15100105
    Avian Leukosis Subgroup A 15100102
    Avian Leukosis Subgroup B 15100103
    Avian Leukosis Subgroup J 15100104
    Chicken Anemia Virus (Minimum of 10 samples) 15100106
    Mycoplasma synoviae and gallisepticum 15100107
    Reticuloendotheliosis 15100108
    Salmonella pullorum 15100109
  • ELISA

    Antibody Detection

    Please provide 0.1 ml chicken serum per test. There is a 30 sample minimum per pathogen for ELISA testing.
    Pathogen Test #
    Avian Encephalomyelitis 15100003
    Avian Influenza 15100004
    Avian Reovirus 15100006
    Avian Rhinotracheitis Virus 15100007
    Chicken Anemia Virus 15100008
    Infectious Bronchitis 15100009
    Infectious Bursal Disease 15100010
    Infectious Laryngotracheitis 15100011
    Newcastle Disease 15100014

    Antigen Detection

    Please provide cloacal swabs, albumen, or 0.5 ml of supernatant from passaged CEFs.
    Pathogen Test #
    Avian Leukosis (p27 antigen detection) 15100005
  • Other Tests
    Please provide 1.0 ml per sample.
    Testing Service Test #
    Avian Encephalomyelitis Flock Susceptibility 15100082
    COFAL (Complement Fixation for Avian Leukosis) 10-day test 15100060
    Salmonella pullorum microtiter 15100017
    Vaccine Potency Test - Single Vaccine 15100064
    Virus Titration 15100065
    Virus Isolations 15100066