Industry-Leading Analytical Capability Comes Online in Edinburgh

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Orbitrap Fusion Lumos

As part of our continued drive for quality and market-leading scientific excellence, Charles River is excited to announce its investment in a new flagship high resolution LC-MS system at its Edinburgh facility.

Managed by a dedicated team of high resolution mass spectrometrists, the combination of a Thermo Scientific™ Vanquish™ UHPLC, and Fusion™ Lumos™ Tribrid™ high resolution spectrometer offers unrivalled sensitivity, resolution, and an array of powerful new experiments, which will be used to provide detailed structural information and to quantify very low concentration analytes.

The two-dimension capable Vanquish™ UHPLC allows rapid online isolation, concentration, and cleanup of peaks from a variety of complex sample types, from plant, animal, and environmental fate through to in vitro studies.

The mass spectrometer has also been upgraded beyond the base model Fusion Lumos™ to allow flexibility around assay type, and to provide a toolbox of valuable functions for the analyst to draw upon when required, including:

  • Ultra-high resolution (1,000,000 FWHM at m/z 200) for in-depth characterization of an expansive range of analytes.
  • Ultraviolet photo-dissociation (UVPD) to aid fragmentation of the most challenging molecules. This unique technique compliments the standard HCD/CID and MSn fragmentation modes and, through use of laser technology, allows in-depth structural analysis of both large and small molecules.
  • Easy-IC (Internal Calibration), which significantly improves the mass-to-charge ratio assignment accuracy to less than 1 ppm. This is achieved through supply of a regulated number of calibrant ions from an embedded secondary source, which are used to provide real-time fine adjustment of the instrument’s mass accuracy. This enables correction of otherwise uncompensated errors that are due to temperature changes and scan-to-scan variations in the total charge of the ion population.

To extend ‘small molecule’ workflows from injection to identification, the latest metabolism software, Compound Discoverer 3.0, streamlines the process of identifying structures, the determination of real differences between samples, and assists with the elucidation of biological pathways. Ultimately, this will compress existing timelines for analysis and production of reports for our clients.

This industry-leading combination of investment, coupled with the expertise of the high resolution mass spectrometry team, provides access to datasets that truly push the boundaries of what is achievable using mass spectrometry for agrochemical and pharmaceutical product development.

If you would like to take advantage of this enhanced capability, we would be delighted to set up a time to talk over your needs. Contact us at [email protected].