Optimizing IHC Staining Through Automation

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immunohistochemistry stain

Charles River recently acquired a Thermo Scientific™ Lab Vision™ Autostainer 720, the industry standard for immunohistochemistry (IHC) automation, enhancing our IHC capabilities through increased speed, efficiency and reproducibility to meet your non-GLP and GLP study needs. The machine has an 84-slide capacity – more than any other autostainer in the industry – allowing for a greater volume of slides to be run at one time. This decreases both run times and the risk for batch variation. Not only does the instrument increase reliability, but it also provides a vast range of flexibility in the types of markers that can be stained. Its completely open system allows for the universal use of reagents in any order desired. Once a marker is qualified, it is saved in a database and can easily be called up when needed. We continue to grow our library of qualified methods for a wide range of markers in various species.

IHC is a valuable quantitative pathology tool that provides visual evidence of the distribution and locations of specific proteins or biomarkers within tissue. At Charles River, we use the instrument for routinely performed IHC staining for studies such as those involving T cell receptors, including CD4 and CD8 among others. It is also a valuable time-saving tool for more unique studies where there is an interest in stains for multiple markers; this is done by enabling slide staining for more than one marker at one time, as opposed to manual staining which often requires individual markers to be split up and stained at different times. Despite the benefits the autostainer brings to a study or program, IHC can be quite complex to perform. It is important to choose a partner who has vast experience and deep scientific knowledge of this technique to avoid compromised study designs and delayed timelines.