Flow cytometry applications and techniques have increasingly been used to rapidly identify and characterize cell type, morphology, and function with statistical power to support the safety and efficacy of new test compounds. However, there are often limitations in certain animal species where blood volume is limited.
The standard three laser cytometer detects up to eight molecules while the five laser system offers clients enhanced multiplex capabilities allowing the detection of up to 18 different molecules of interest within one test and improves compensation on 10 to 18 color panels.
Charles River Laboratories has upgraded its BD LSRFortessa™ X-20 flow cytometers to a five laser, 18 color configuration to support immunology endpoints in preclinical and clinical drug development. The flexibility in laser wavelengths allows researchers to optimize their assay design by selecting the latest fluorescent dyes and antibodies required within experimental protocols.
Investment in such technologies provides sponsors with a greater choice at lower cost and reduced sample volume. It provides higher, faster throughput, produces less waste, and translates into a reduced cost per sample to the sponsor.
The next few years will be as exciting as the last few with the further development of immunomodulators and biotherapeutics such as mAb fragments, bispecific mAbs, biosimilars, and antibody drug conjugates. The demand in flow cytometry continues to grow and Charles River is assisting sponsors to incorporate this essential technique as both an in vitro and in vivo diagnostic tool. Check out our infographic on the top five tips to set up and run any flow cytometry assay.